ArticlePoultry science2026
A chicken microRNA expressing LaSota targets Newcastle disease virus phosphoprotein and impacts interferon expression.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Newcastle disease (ND), a major cause of economic loss in the poultry industry worldwide, is caused by outbreaks of virulent avian paramyxovirus type 1(APMV-1) virus. We previously demonstrated that chicken miRNA-27b-5p (gga-miR-27b-5p) can regulate the APMV-1 phosphoprotein (P) and alter viral replication. In this study, the LaSota vaccine strain of APMV-1 was used as a backbone to express the chicken pre-miRNA-27b and expected to increase intra-cellular levels of both gga-miR-27b-5p and gga-miR-27b-3p. This novel recombinant virus, rLS-miR-27b, was rescued, propagated in embryonating chicken eggs (ECEs) and did not exhibit toxicity in ECEs. Expression of both gga-miR-27b-5p and gga-miR-27b-3p was increased significantly relative to controls. An inverse correlation was observed between increased expression of gga-miR-27b-5p and reduced APMV-1 P transcripts. Additionally, increased expression of interferons (alpha, beta and lambda) and interferon stimulated gene MX1 was observed. Suppression of P did not alter the P:V ratio in rLS-27b virus infected cells. These data support the observation that miR-27b-5p expression alters viral transcriptional kinetics in cell culture without affecting overall progeny virus yield in ECEs and could be utilized to improve current APMV-1 vaccines.
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