ArticlePloS one2026
Relationship between neonatal glutamine supplementation and amino acid profiles, protein synthesis in splanchnic tissues and the plasma metabolome in 5-day-old piglets with low birth weight.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidence of low birthweight (LBiW) piglets, which show impaired growth compared to normal birthweight (NBiW) littermates, has increased with litter size. We previously reported that glutamine increased the bodyweight and plasma glutamine concentrations in LBiW piglets, with no effect on jejunal glutamine concentrations or glutamine transport and metabolism mRNA abundance. In this study, we investigated the effect of glutamine supplementation on proteinogenic free amino acid (FAA), protein-bound AA (PBAA) and AA-metabolite profiles, mRNA abundance, fractional protein synthesis (FPSR) and the plasma metabolome. At birth (0 days of age (d)), 24 pairs of male LBiW (1.10 kg ± 0.02) and NBiW (1.50 kg ± 0.02) littermates were selected. At 1 d, litters were standardized to 12 piglets, and piglets supplemented daily with either glutamine (1 g/kg bodyweight) or isonitrogenous amounts of alanine (1.22 g/kg bodyweight), until 5 d. Stomach content FAA, AA-metabolites, duodenal and liver tissue FAA, PBAA, AA-metabolites, biochemical indices, duodenal, colon and liver tissue FPSR, liver tissue and duodenal mucosa glutamine transport and metabolism mRNA abundance and the plasma metabolome were analysed. Stomach digesta glutamine (P < 0.001) and glutamate (LBiW, P = 0.02; NBiW, P = 0.03) concentrations were higher in glutamine than alanine piglets. Analysis by Data Integration Analysis for Biomarker Discovery using Latent Variable Approaches for Omics Studies showed glutamine, carnosine, citrulline and alanine separated the supplementation groups, whilst glutathione reductase, glutathione synthetase and tight junction protein 1 mRNA separated glutamine LBiW piglets from alanine. Duodenal RNA was lower and Protein-to-RNA ratio higher (P = 0.004) in glutamine than alanine LBiW piglets (P = 0.02). Colon FPSR was higher in NBiW than LBiW piglets supplemented with Ala. No effects on the plasma metabolome were observed. These results show that not all glutamine passed through the stomach, and that glutamine is absorbed and metabolised in the duodenum, potentially altering the intracellular pH, and appears to increase oxidative stress markers in the duodenum of LBiW piglets.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.