Evidence map›Paper›PMID 42862238›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Native Liquid MALDI (NALIM) Mass Spectrometry Analysis of RNA-Binding Protein Complexes.

Edison Zhamungui, James Irving, Martine Beaufour, Martine Cadene

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Edison ZhamunguiUniversité d'Orléans, Orléans, France.ORCID http://orcid.org/0009-0001-8269-4856
James IrvingUCL Respiratory and the Institute of Structural and Molecular Biology, University College London, London, UK.ORCID http://orcid.org/0000-0003-3204-6356
Martine BeaufourUniversité d'Orléans, Orléans, France.ORCID http://orcid.org/0009-0004-9916-0545
Martine CadeneUniversité d'Orléans, Orléans, France. martine.cadene@cnrs-orleans.fr.ORCID http://orcid.org/0000-0003-4936-2662

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Native mass spectrometry (nMS) techniques are designed to preserve non-covalent biomolecular complexes for direct observation in an MS instrument. In the past few years, nMS based on NALIM (NAtive LIquid MALDI) has been developed to complement established ESI-based nMS approaches, with the aim to tackle large or poorly soluble protein complexes. It affords a straightforward reading of spectra to monitor oligomer distributions, with applications ranging from the determination of protomers stoichiometry to the presence of peptide or oligonucleotide ligands in complexes. It is well suited to the analysis of helicases and other protein complexes involved in nucleic acid transactions.

Indexed as

RNA-Binding ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationLigandsProtein BindingLigandsRNA-Binding ProteinsBiomolecular complexesLiquid matrixMALDINative MALDINative mass spectrometryNoncovalent complexesNondenaturing conditionsProtein–ligand interactionsProtein–protein interactions

Identifiers

PMID42862238

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.