Evidence map›Paper›PMID 42862236›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Insights into the Activities of Helicases from Thermococcales: The Cases of the RNA Helicase ASH-Ski2, the DNA Helicase Hel308, and the RNA:DNA Helicase Lhr2.

Marion Tassoni, Manon Batista, Mirna Hajj, Béatrice Clouet-d'Orval, Marie Bouvier

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Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marion TassoniCNRS, University of Toulouse, CBI, Toulouse, France.
Manon BatistaCNRS, University of Toulouse, CBI, Toulouse, France.
Mirna HajjCNRS, University of Toulouse, CBI, Toulouse, France.
Béatrice Clouet-d'OrvalCNRS, University of Toulouse, CBI, Toulouse, France.ORCID http://orcid.org/0000-0002-3591-8538
Marie BouvierCNRS, University of Toulouse, CBI, Toulouse, France. marie.bouvier@utoulouse.fr.ORCID http://orcid.org/0000-0002-2111-052X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Helicases are versatile enzymes. Superfamily 2 (SF2) is the largest and most diverse group of helicases. SF2 helicases are involved in fundamental processes dealing with nucleic acids in the three domains of life and can be specific to DNA, RNA, or DNA:RNA hybrids. They are known to catalyze unwinding reactions in vitro or disrupt duplexes in vivo. More recently, it has emerged that they can also anneal single strands to form duplexes. Here, we describe biochemical assays used to investigate the activities of the Ski2-like helicases ASH-Ski2 and Hel308, as well as the Lhr-like helicase Lhr2 from Thermococcales, an order of hyperthermophilic archaea. Unwinding and strand-annealing assays using a range of substrates revealed that they are RNA-, DNA-, or RNA:DNA-specific helicases, respectively.

Indexed as

Archaeal ProteinsDNA HelicasesRNA HelicasesAdenosine TriphosphateDNARNAAdenosine TriphosphateArchaeal ProteinsDNADNA HelicasesRNARNA HelicasesArchaeaATP hydrolysisHelicase activityHyperthermophileLhr-like helicaseNucleic acid bindingSki2-like helicase

Identifiers

PMID42862236

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.