Evidence map›Paper›PMID 42861354›Full record

ArticleCurrent research in food science2026

Integrated computation-guided thermostabilization of the fungal sweet protein Honey Truffle Sweetener.

Zhikuan Wang, Wen Wang, Feifei Zhu, Yutian Zhang, Yirui Li, Zhiqi Zhu, Ziwan Lu, Ang Huang, Mingjia Yu, Si Liu

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Article in Current research in food science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Zhikuan WangState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Wen WangKey Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
Feifei ZhuState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Yutian ZhangState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Yirui LiState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Zhiqi ZhuState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Ziwan LuState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Ang HuangDepartment of Gastroenterology and Hepatology, The First Medical Center of PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China.
Mingjia YuKey Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing, 100081, China.
Si LiuState Key Laboratory of Synthetic Biology, Haihe Laboratory of Sustainable Chemical Transformations, Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, Faculty of Medicine, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thermostability limits the use of sweet proteins as sugar substitutes in thermally processed foods. Here, we report an integrated computation-guided workflow for thermostabilizing Honey Truffle Sweetener (HTS), a recently identified fungal sweet protein with low intrinsic stability. We determined the HTS crystal structure and used it to guide subsequent computational analyses. Rosetta and ThermoMPNN evaluated candidate substitutions from complementary physics-based and deep-learning-based perspectives, and the selected single-mutant library was experimentally characterized. The resulting melting-temperature data were then used to develop SMRC-Net, which combines mutation-centered features from the ESM2 protein language model with molecular dynamics-derived structural descriptors to support mutation prioritization. Repeated nested cross-validation supported quantitative T

Indexed as

Computational protein designHoney truffle sweetenerMolecular dynamicsProtein engineeringSweet proteinThermostability

Identifiers

PMID42861354
PMCPMC13654475

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