Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis.

Rikuto Oishi, Ryunosuke Katayama, Mitsuhiro Ueda, Tatsuji Sakamoto, Masami Nakazawa

Abstract read

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Article in The Journal of eukaryotic microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Rikuto OishiDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Japan.

Ryunosuke KatayamaDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Japan.

Mitsuhiro UedaDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Japan.

Tatsuji SakamotoDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Japan.

Masami NakazawaDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Japan.ORCID https://orcid.org/0000-0003-1639-0560

Funding

Japan Science and Technology Agency GteX Program Japan grant no. JPMJGX23B0Japan Science and Technology Agency SPRING JPMJSP2139Japan Society for the Promotion of Science KAKENHI grant no. JP24K01897

6 · The paper itself

Abstract

Under anaerobic conditions, Euglena gracilis degrades paramylon and synthesizes wax esters (WEs) while accumulating succinate in the culture supernatant. Succinate can either be converted to succinyl-CoA by succinyl-CoA synthetase (SCS) and enter the propionyl-CoA biosynthetic pathway or accumulate extracellularly. Propionyl-CoA serves as a primer for odd-chain WE synthesis. Prior research revealed that knockout (KO) of the SCS α-subunit (SCSα) nearly eliminated odd-chain WE synthesis while preserving overall WE accumulation. In the present study, the effects of targeted modifications at anaerobic metabolic branch points on carbon allocation between WE synthesis and succinate accumulation were examined. SCSα KO increased succinate accumulation and markedly reduced odd-chain WEs while largely maintaining total WE levels. Silencing mitochondrial NADP

Indexed as

CarbonEstersEuglena gracilisMetabolic EngineeringSuccinic AcidWaxesAcyl Coenzyme AAnaerobiosisGlucansMalate DehydrogenaseSuccinate-CoA LigasesAcyl Coenzyme ACarbonEstersGlucansMalate DehydrogenaseparamylonSuccinate-CoA LigasesSuccinic Acidsuccinyl-coenzyme AWaxesanaerobic metabolismcarbon allocationEuglena gracilismalic enzymeodd‐chain fatty acid biosynthetic pathwaysuccinate metabolismsuccinyl‐CoA synthetasewax ester

Identifiers

PMID42860623

DOI10.1111/jeu.70134

PMCPMC13655293

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.