Evidence map›Paper›PMID 42860608›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

One-Step Induction of Oogonia-Like and Pro-Spermatogonia-Like Cells From Porcine Pluripotent Stem Cells.

Pengcheng He, Liang Zhong, Tianzhi Chen, Yaxuan Yan, Xinze Chen, Runbo Li, Yingjie Wang, Yadi Teng, Minglei Zhi, Meng Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Pengcheng He *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.ORCID https://orcid.org/0009-0001-0497-721X
Liang Zhong *Hebei Key Laboratory of Basic Medicine for Diabetes, Shijiazhuang Second Hospital, Hebei, China.ORCID https://orcid.org/0000-0001-5419-4603
Tianzhi Chen *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.ORCID https://orcid.org/0000-0002-2481-6448
Yaxuan YanState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Xinze ChenState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Runbo LiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Yingjie WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Yadi TengState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Minglei ZhiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Meng WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Jinying ZhangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.
Fan ZhaoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, China.
Dengfeng GaoState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
Suying CaoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, China.
Jianyong HanState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), College of Biological Sciences, China Agricultural University, Beijing, China.ORCID https://orcid.org/0000-0002-9549-3279

Funding

Biological Breeding-National Science and Technology Major Project 2023ZD0407503China Postdoctoral Science Foundation 2023M743802China Postdoctoral Science Foundation 2026T190069Hebei Medical Science Research Project 20210179Hebei Traditional Chinese Medicine Research Project 2022186National Key R&D Program of China 2022YFD1302201Natural Science Foundation of China 32302746 32370846Pinduoduo-China Agricultural University Research Fund PC2024A01001
6 · The paper itself

Abstract

Pigs share a germ cell regulatory network highly similar to that of humans, yet lack a robust system for inducing and expanding germ cells from pluripotent stem cells, impeding large-animal gametogenesis research. Here, we established a modular platform capable of efficiently inducing and long-term expanding porcine germ cell-like cells directly from pluripotent stem cells. We first developed a one-step method to induce pro-spermatogonia-like cells (activating SOX17) and oogonia-like cells (activating SOX17/TFAP2C) from porcine pre-gastrulation epiblast stem cells (pgEpiSCs) harboring a NANOS3-tdTomato (NT) reporter, via activation of exogenous transcription factors. Single-cell transcriptome profiling revealed a differentiation trajectory from pgEpiSCs through primordial germ cell-like cells (PGCLCs) to pro-spermatogonia/oogonia-like cells, with concomitant differentiation of gonadal somatic cells. Functional analyses demonstrate that EGF activates core PGC network and is essential for porcine PGCLCs survival in a xenogeneic reconstituted ovary, BMP4 drives NANOS3 and DDX4 expression, and IWR1 enhances germ cell differentiation by suppressing mesodermal programs. Finally, to address the challenge of cell quantity, we established a long-term expansion module based on FGF2, IWR1, and EGF that sustains NT-positive cells for up to 120 days while preserving their early PGC and pro-spermatogonia-like molecular characteristics. Our study provides new insights into porcine gametogenesis and reproductive development across species.

Indexed as

gametogenesisoogoniapluripotent stem cellsporcineprimordial germ cellspro‐spermatogonia

Identifiers

PMID42860608
PMCPMC13655266

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.