Evidence map›Paper›PMID 42859277›Full record

ArticleBiodegradation2026

Biochemical and molecular insights into the antibiofilm and antivirulence effects of a B-phycoerythrin-rich extract from Porphyridium cruentum against Pseudomonas aeruginosa.

Oumaima Ben Soltana, Mohamed Barkallah, Taheni Bouaziz, Alain Dufour, Fatima El Garah, Slim Abdelkafi, Imen Fendri

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Article in Biodegradation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Oumaima Ben SoltanaLaboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, 3038, Sfax, Tunisia.
Mohamed BarkallahLaboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, 3038, Sfax, Tunisia.
Taheni BouazizLaboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, 3038, Sfax, Tunisia.
Alain DufourEMR CNRS 6076, IUEM, Université Bretagne Sud, 56100, Lorient, France.
Fatima El GarahLGC, Université Toulouse, Toulouse INP, CNRS, Toulouse, France.
Slim AbdelkafiLaboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, 3038, Sfax, Tunisia. slim.abdelkafi@enis.tn.ORCID https://orcid.org/0000-0003-1314-0523
Imen FendriLaboratory of Plant Biotechnology Applied to Crop Improvement (LR01ES21), Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The growing resistance of Pseudomonas aeruginosa to conventional antibiotics has drawn increasing attention to antivirulence strategies that inhibit biofilm development and interfere with Quorum Sensing (QS) mechanisms. This study evaluated the biological activity of B-phycoerythrin (B-PE), a phycobiliprotein extracted from the red microalga Porphyridium cruentum, against Pseudomonas aeruginosa PAO1. This phycobiliprotein significantly inhibited biofilm formation by up to 62.4% and reduced performed biofilm by over 63% at 1/2 MIC, as quantified by crystal violet staining, causing notable morphological changes observed via epifluorescence microscopy. Furthermore, B-PE affected the production of virulence factors, decreasing pyocyanin and pyoverdine synthesis and reducing cell surface hydrophobicity. RT-qPCR analysis revealed significant downregulation of lasI, lasR, rhlI, rhlR, and mvfR, indicating modulation of multiple QS-associated regulatory components. These transcriptional changes, together with the observed alterations in QS-associated phenotypes, suggest that QS-associated regulation may contribute to the biological effects of the B-PE-rich extract. Overall, this study provides evidence for the antibiofilm and antivirulence potential of the B-PE-rich extract against Pseudomonas aeruginosa, supporting its further investigation as a naturally derived preparation for biofilm control and anti-infective applications.

Indexed as

Anti-Bacterial AgentsBiofilmsPhycoerythrinPlant ExtractsPorphyridiumPseudomonas aeruginosaBacterial ProteinsGene Expression Regulation, BacterialMicrobial Sensitivity TestsQuorum SensingVirulenceVirulence FactorsAnti-Bacterial AgentsBacterial ProteinsPhycoerythrinPlant ExtractsVirulence FactorsBiofilm inhibitionPhycoerythrinPorphyridium cruentumPseudomonas aeruginosaQuorum sensing

Identifiers

PMID42859277

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.