Evidence map›Paper›PMID 42856864›Full record

ArticleVeterinary world2026

Development and validation of a quadruplex real-time quantitative polymerase chain reaction assay for the simultaneous detection of Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Actinobacillus pleuropneumoniae, and Glaesserella parasuis.

Hongyu Wei, Yu Gan, Kaichuang Shi, Yuwen Shi, Feng Long, Shuping Feng, Yanwen Yin, Sujie Qu, Wenjun Lu, Yingyi Wei

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Article in Veterinary world, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hongyu WeiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Yu GanCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Kaichuang ShiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Yuwen ShiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Feng LongGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Shuping FengGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Yanwen YinGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Sujie QuGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Wenjun LuGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Yingyi WeiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Materials and Methods: Four pairs of species-specific primers and TaqMan probes targeting the Results: The optimized quadruplex assay showed excellent linearity, with correlation coefficients (R²) ≥0.998 for all four targets. The limits of detection were 152.02, 158.53, 161.36, and 140.40 copies/reaction for Conclusion: The developed quadruplex real-time qPCR assay provides a rapid, sensitive, specific, and reproducible method for the simultaneous detection of four major bacterial pathogens associated with PRDC. Its excellent diagnostic performance and ability to identify mixed infections make it a valuable tool for routine laboratory diagnosis, epidemiological surveillance, and disease control in pig populations, with potential application in large-scale surveillance programs.

Indexed as

Actinobacillus pleuropneumoniaeGlaesserella parasuismultiplex real-time quantitative polymerase chain reactionMycoplasma hyopneumoniaeMycoplasma hyorhinisporcine respiratory disease complexswineTaqMan probe

Identifiers

PMID42856864
PMCPMC13650294

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.