Evidence map›Paper›PMID 42856545›Full record

ArticlebioRxiv : the preprint server for biology2026

A bifunctional RNase H protein supports DNA replication and transcription in nucleus-forming

Phoolwanti Rani, Niklas Klusch, Shannon M Barret, Taylor Forman, Makaela Levine, Margot Riggi, Caroline Rees, Rui Liu, Lizbeth Y Hoffman, Meline Norquist and 7 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Phoolwanti RaniDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0002-6731-9003
Niklas KluschDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0003-2459-7377
Shannon M BarretHoward Hughes Medical Institute, University of California San Diego, La Jolla, CA 92093.ORCID 0009-0001-9775-7952
Taylor FormanDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0003-4174-4682
Makaela LevineHoward Hughes Medical Institute, University of California San Diego, La Jolla, CA 92093.
Margot RiggiDepartment of Cell and Virus Structure, Max Planck Institute of Biochemistry, Martinsried, Germany.ORCID 0000-0002-8930-5331
Caroline ReesDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0009-0002-8676-2796
Rui LiuDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0002-3758-6493
Lizbeth Y HoffmanDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0009-0008-5718-2323
Meline NorquistDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0009-0002-2835-8293
Dwaipayan BasuDepartment of Biochemistry and Molecular Biophysics, University of California San Diego, La Jolla CA 92093.ORCID 0009-0008-8207-8712
Koe InlowDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0002-2535-9613
Emily G ArmbrusterDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0003-0377-1209
Chase J MorganDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0002-7498-8678
Joe PoglianoDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0001-8989-2655
Kevin D CorbettDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0001-5854-2388
Elizabeth VillaDepartment of Molecular Biology, University of California San Diego, La Jolla CA 92093.ORCID 0000-0003-4677-9809

Funding

Molecular and cellular biology of the phage nucleus and spindleR01GM129245 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI POGLIANO, JOSEPH A · 2018 to 2025
$4.2M
Molecular mechanisms of nucleic acid recognition and maintenance in meiosis and innate immunityR35GM144121 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett · 2022 to 2026
$2.7M
NIGMS NIH HHS R01 GM129245NIGMS NIH HHS R35 GM144121
6 · The paper itself

Abstract

Jumbo bacteriophages of the Chimalliviridae family replicate their genomes within a proteinaceous phage nucleus that spatially segregates viral DNA replication and transcription from translation. While the phage nucleus protects viral DNA from host defenses, it also imposes unique constraints on genome replication and gene expression. Here we identify the phage-encoded protein Rhc (RNase H-clamp) as a central mediator of genome transactions within the phage nucleus. Using the

Identifiers

PMID42856545
PMCPMC13648698

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.