Evidence map›Paper›PMID 42856330›Full record

ArticlebioRxiv : the preprint server for biology2026

Multi-omic spatial mapping of the human habenula defines the molecular and anatomical organization of medial and lateral subregions.

Kelsey D Montgomery, Jonathan M Werner, Nicholas J Eagles, Chunyu Liu, Cynthia S Cardinault, Svitlana V Bach, Atharv Chandra, Ruth Zhang, Sarah E Maguire, Heena R Divecha and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Kelsey D MontgomeryLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0001-8420-0138
Jonathan M WernerLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0001-9248-6420
Nicholas J EaglesLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-9808-5254
Chunyu LiuDepartment of Mental Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.ORCID 0000-0001-6705-7341
Cynthia S CardinaultLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-6877-514X
Svitlana V BachLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0003-1134-9585
Atharv ChandraLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0009-0000-3019-7308
Ruth ZhangLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0009-0003-2134-0952
Sarah E MaguireLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0009-0005-2884-4787
Heena R DivechaLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-1959-0675
Amy Deep-SoboslayLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-7762-8942
Ryan A MillerLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0003-3477-7443
Louise A Huuki-MyersLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0001-5148-3602
Rahul A BharadwajLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-7749-8682
Joel E KleinmanLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-4210-6052
Thomas M HydeLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0002-8746-3037
Brion MaherDepartment of Mental Health, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.ORCID 0000-0001-9809-0064
Leonardo Collado-TorresLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0003-2140-308X
Kristen R MaynardLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, 21205, USA.ORCID 0000-0003-0031-8468

Funding

Vaccine Response and Immunotherapeutics SWGP30AI094189 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Richard E. Chaisson · 2012 to 2026
$67.0M
Regulation of Gene Expression in the Human Habenula in Comorbid Opioid Addiction and DepressionR01DA055823 · NIDA · LIEBER INSTITUTE, INC. · PI Kristen Rose Maynard · 2022 to 2026
$3.3M
MoFlo to MoFlo XDP Upgrade for BSL3 SortingS10OD016315 · OD · JOHNS HOPKINS UNIVERSITY · PI MARGOLICK, JOSEPH B. · 2013 to 2013
$233k
NIAID NIH HHS P30 AI094189NIDA NIH HHS R01 DA055823NIH HHS S10 OD016315
6 · The paper itself

Abstract

The habenula (Hb) is a small epithalamic midline structure critical for reward processing and mood regulation that is implicated in many complex brain disorders, including depression and addiction. The molecular anatomy of the Hb has been well-characterized in model organisms, but little is known about its organization in the human brain, including the transcriptomic and epigenetic signatures of spatially-organized lateral (LHb) and medial (MHb) cell types. Here, we generated a high resolution spatiomolecular map of the human Hb using both multiomic single nucleus sequencing (snMultiome) and spatially-resolved transcriptomics. We measured gene expression and chromatin accessibility across 4 MHb and 5 LHb neuronal cell types as defined by transcriptomic conservation with rodents and zebrafish. We identified cell type-specific molecular specializations related to GABAergic and glutamatergic signaling as well as regulatory relationships between open chromatin regions, transcription factors, and target genes associated with depression and addiction. High resolution spatial transcriptomics identified the anatomical location of heterogeneous LHb subpopulations, including two putative inhibitory populations in the LHb. We further characterized cell-type relationships between Hb neurons and glial subtypes and predicted ligand-receptor interactions between LHb and MHb neurons. Finally, we delineated Hb cell types and spatial domains enriched in genes associated with genetic risk for psychiatric disorders, identifying the strongest enrichment in the MHb. Collectively, the generated data and analyses resolve the spatiomolecular organization of the human Hb and identify molecular-genetic associations with neuropsychiatric disease.

Identifiers

PMID42856330
PMCPMC13648681

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