Evidence map›Paper›PMID 42855727›Full record

ArticleZoological letters2026

Resolving species boundaries in Mediterranean Xiphinema (Nematoda: Longidoridae) through multivariate morphometrics and multilocus profiling: four new species and two cryptic complexes.

Rosana Salazar-García, Juan E Palomares-Rius, Carolina Cantalapiedra-Navarrete, El Amine Ajal, Víctor Lechuga, Inmaculada Criado-Navarro, Pablo Castillo, Antonio Archidona-Yuste

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Article in Zoological letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Rosana Salazar-GarcíaDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
Juan E Palomares-RiusDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
Carolina Cantalapiedra-NavarreteDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
El Amine AjalUPR of Pharmacognosy, Faculty of Medicine and Pharmacy of Rabat, Mohammed V University, BP 6203, Rabat, 1000, Morocco.
Víctor LechugaDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
Inmaculada Criado-NavarroDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
Pablo CastilloDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain.
Antonio Archidona-YusteDepartment of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Córdoba, 14004, Spain. antonio.archidona@ias.csic.es.ORCID http://orcid.org/0000-0003-4446-0642

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a major biodiversity hotspot, the Mediterranean Basin provides an ideal setting for advancing our understanding of the diversity patterns of plant-parasitic nematodes. The genus Xiphinema is notably diverse in Mediterranean agroecosystems, yet species delimitation remains challenging due to the presence of cryptic lineages. Here, we address this issue by integrating morphology, morphometrics, and molecular data to characterise 50 Xiphinema populations from 264 sampling sites across agricultural landscapes and natural habitats in the main olive-growing regions of the Mediterranean Basin. Our findings yielded four major findings: (i) identification of four new species, X. andalusomarocense sp. nov. X. africana sp. nov. X. alentejanum sp. nov. and X. cryptoduriense sp. nov.; (ii) discovery of two well-supported cryptic complexes by combining multivariate morphometric analyses, multilocus molecular profiling, and phylogenetic inference (X. baetica- and X. duriense-complexes); (iii) demonstration of the diagnostic value of morphological variation in size and shape for delimiting species within cryptic complexes; and iv) the generation of new ribosomal and mitochondrial sequences for 18 Xiphinema species, expanding the molecular resources available for this group. Together, these results refine the systematics of Xiphinema, broaden our understanding of nematode diversity in the Mediterranean Basin, and provide a robust framework for future evolutionary research on Xiphinema.

Indexed as

COI mtDNAD2-D3 of 28S rRNAITS1 rRNAMorphometrics analysesPartial 18S rRNASoil nematodesSpecies delimitation

Identifiers

PMID42855727
PMCPMC13653414

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