Evidence map›Paper›PMID 42853419›Full record

ArticlePlanta2026

Comprehensive analysis of the function of the GolS family and LpGols7 in Lespedeza potaninii under drought stress.

Xue Li, Lincheng Cai, Fuhui Shi, Bike Su, Rui Hu, Tianbao Zang, Jiyu Zhang, Qi Yan

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Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Xue LiState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Lincheng CaiState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Fuhui ShiState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Bike SuState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Rui HuState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Tianbao ZangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Jiyu ZhangState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China. zhangjy@lzu.edu.cn.
Qi YanState Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China. yanqi@lzu.edu.cn.ORCID http://orcid.org/0000-0001-6413-2385

Funding

National Natural Science Foundation of China 32301478
6 · The paper itself

Abstract

MAIN

conclusionSeven LpGolS genes were identified in Lespedeza potaninii and showed tissue-specific expression and drought induction. Overexpression of LpGolS7 improves drought tolerance. Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs) that plays a crucial role in mediating plant responses to abiotic stress. However, the evolutionary relationships and biological functions of the GolS family in xerophytic Lespedeza potaninii remain unclear. In this study, we identified seven members of the GolS gene family at the whole-genome level in L. potaninii. Phylogenetic analysis revealed that the GolS family members from 18 plants could be divided into 4 subfamilies. Groups I, II, and III were mainly composed of dicotyledonous genes, while Group IV was dominated by monocotyledonous genes. Expression profiling analysis revealed a specific LpGolS gene expression phenomenon, in which they were highly expressed in the roots and seeds. Transcriptomic and qRT-PCR analyses revealed that the expression levels of LpGolS genes in the shoots and roots of L. potaninii were significantly induced by drought stress. These findings indicate that LpGolS genes play important roles in the drought response of L. potaninii. Heterologous expression analysis indicated that the heterologous expression of LpGolS4 and LpGolS7 enhanced yeast tolerance to polyethylene glycol (PEG) stress. Subcellular localisation analysis revealed that GFP (Green fluorescent protein)-LpGolS7 fusion protein expression was distributed throughout both the nucleus and the cytoplasm. Overexpression of LpGolS7 improved the drought tolerance of L. potaninii by increasing the soluble sugar content. These results provide a foundation for subsequent functional analysis of the GolS gene in L. potaninii.

Indexed as

GalactosyltransferasesPlant ProteinsDrought ResistanceDroughtsGene Expression ProfilingGene Expression Regulation, PlantMultigene FamilyPhylogenyPlant RootsPlants, Genetically ModifiedRaffinoseStress, Physiologicalgalactinol -raffinose galactosyltransferaseGalactosyltransferasesinositol 1-alpha-galactosyltransferasePlant ProteinsRaffinoseEvolutionary analysisExpression patternGalactinol synthaseNative grass

Identifiers

PMID42853419

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.