Evidence map›Paper›PMID 42852455›Full record

ArticleNature photonics2026

Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.

Zhaoqiang Wang, Ruth R Sims, Sheng Xiao, Ruixuan Zhao, Ohr Benshlomo, Zihan Zang, Jiamin Wu, Valentina Emiliani, Liang Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature photonics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhaoqiang WangDepartment of Bioengineering, University of California, Los Angeles, CA, USA 90095.
Ruth R SimsInstitut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France 75012.
Sheng XiaoChan Zuckerberg Biohub, San Francisco, CA, USA 94158.
Ruixuan ZhaoDepartment of Bioengineering, University of California, Los Angeles, CA, USA 90095.
Ohr BenshlomoDepartment of Bioengineering, University of California, Los Angeles, CA, USA 90095.
Zihan ZangDepartment of Bioengineering, University of California, Los Angeles, CA, USA 90095.
Jiamin WuDepartment of Automation. Tsinghua University, China 100084.
Valentina EmilianiInstitut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France 75012.
Liang GaoDepartment of Bioengineering, University of California, Los Angeles, CA, USA 90095.

Funding

Ultrafast BioimagingR35GM128761 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Liang Gao · 2018 to 2026
$3.2M
Kilohertz 3D Optical Mapping of Atrial Fibrillation in Beating Zebrafish HeartsR01HL165318 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, HSIAI, TZUNG K · 2022 to 2025
$2.2M
Red-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imagingR01NS136027 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2024 to 2026
$1.7M
Kilohertz volumetric imaging of neuronal action potentials in awake behaving miceRF1NS128488 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, GOLSHANI, PEYMAN · 2022 to 2022
$1.6M
NHLBI NIH HHS R01 HL165318NIGMS NIH HHS R35 GM128761NINDS NIH HHS R01 NS136027NINDS NIH HHS RF1 NS128488
6 · The paper itself

Abstract

Electrical signals in neurons underlie perception, movement, memory, and behaviour, yet many unfold too rapidly to be captured by conventional optical imaging. Calcium imaging has transformed neuroscience but provides an indirect and relatively slow readout of electrical activity. By directly measuring membrane-potential changes, voltage imaging enables millisecond-scale recording of action potentials, subthreshold dynamics, and signal propagation across neural circuits. Recent advances in voltage-sensitive dyes and genetically encoded voltage indicators have made voltage imaging increasingly practical, motivating the development of fluorescence microscopy methods optimized for high-speed acquisition. However, voltage imaging remains constrained by trade-offs among imaging speed, spatial resolution, signal-to-noise ratio, and photodamage. In this Review, we discuss high-speed optical microscopy strategies that address these challenges and highlight the need for co-design among voltage indicators, imaging systems, and computational analysis.

Indexed as

high-speed optical microscopyneural imagingvoltage imaging

Identifiers

PMID42852455
PMCPMC13649331

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.