ArticlePeerJ2026
Comparative evaluation of culture systems for shoot propagation and phytochemical characteristics in pokeweed (
Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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5 authors.
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Abstract
Background: Methods: Nodal explants were cultured on solid and liquid media or in a TIS bioreactor. Six TIS regimes combining 5 or 10 min immersion durations with 1, 2, or 3 immersions per day were evaluated. Growth, callus formation, biomass, total phenolic content (TPC), total flavonoid content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity were determined. Multivariate analyses were used to characterize treatment responses. Results: All treatments showed 100% explant survival and response. Liquid culture produced the weakest shoot and leaf development, whereas TIS generally supported greater shoot growth. T6 (10 min, three immersions/day) produced the highest shoot length (5.69 ± 0.83 cm), leaf length (4.79 ± 0.55 cm), and dry weight (0.12 ± 0.03 g). T5 produced the highest TPC (14.82 ± 0.10 mg gallic acid equivalents per gram (GAE/g) extract), solid culture the highest TFC (31.25 ± 0.94 mg quercetin equivalents per gram (QE/g) extract), and T1 the strongest DPPH radical-scavenging activity (IC Conclusion: Culture system and immersion regime differentially influenced the growth and phytochemical characteristics of
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