ArticleIranian journal of veterinary research2026
Mito-TEMPO improves preservation of cooled canine semen over a 72-hour period of storage.
Article in Iranian journal of veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: The positive effect of Mito-TEMPO, as a mitochondria-targeted antioxidant, on semen preservation has been reported in various species; however, little information is available on canine semen in this regard. Aims: The present study was conducted to evaluate the effect of Mito-TEMPO on preservation of cooled canine semen over a 72 h period of storage. Methods: In study I, the effect of 0 (as control), 25, 50 and 100 μM concentrations of Mito-TEMPO were evaluated on sperm motility, morphology, viability and plasma membrane integrity at time points 0, 24, 48 and 72 h. In study II, the effect of 0 (as control) and 100 μM (as the most effective dose recognized in study I) concentration of Mito-TEMPO was assessed on reactive oxygen species (ROS) and mitochondrial membrane potential at time points 0 and 72 h. Results: In study I, motility, velocity, viability, and proportion of sperm with normal morphology and intact plasma membrane decreased in all groups over time (P<0.05), but this decrease was slower in sperm treated with different doses of Mito-TEMPO than untreated sperm (P<0.05). In this regard, decrease in total and progressive motility observed from 24 h onward in the untreated group; however, such decrease was detected form 48 h onward in groups treated with different doses of Mito-TEMPO (P<0.05). Although all doses of Mito-TEMPO were effective, treatment with 100 μM concentration of Mito-TEMPO provided the best preservation as compared with the control group (P<0.05). Furthermore, study II revealed that ROS generation increased and mitochondrial membrane potential decreased over time in both groups (P<0.05); however, treatment with 100 μM concentration of Mito-TEMPO ameliorated the increase in ROS and the decrease in mitochondrial membrane potential over time (P<0.05). Conclusion: Mito-TEMPO enhances structural and functional characteristics of canine sperm preserved at 4°C for 72 h, and could serve as an effective antioxidant in canine semen extender.
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