Evidence map›Paper›PMID 42851758›Full record

ArticleComputational and structural biotechnology journal2026

Unbiased Long-Read Whole-Genome Sequencing Enables High-Resolution Mapping of Transgene Concatenation and Off-target Genomic Disruption in a Mouse Model.

Maanik Mehta, Kashif Ahmed, Rowaida Hussein, Erika Tavares, Zorana Berberovic, Rabiat Adele, Abigail D'Souza, Bin Gu, Michael D Wilson, Evgueni Ivakine and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Computational and structural biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Maanik MehtaGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0009-0003-9831-2714
Kashif AhmedGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0001-7370-1148
Rowaida HusseinGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0009-0009-7450-6921
Erika TavaresGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0002-5714-1766
Zorana BerberovicThe Centre for Phenogenomics, Toronto, ON M5T 3H7, Canada.
Rabiat AdeleGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0009-0004-2539-5341
Abigail D'SouzaThe Centre for Phenogenomics, Toronto, ON M5T 3H7, Canada.ORCID https://orcid.org/0000-0002-9748-5088
Bin GuDepartment of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA.ORCID https://orcid.org/0000-0002-5594-2463
Michael D WilsonGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0002-4015-3066
Evgueni IvakineGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0003-2132-4699
Philippe P MonnierDepartment of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3K3, Canada.ORCID https://orcid.org/0000-0002-6221-4130
Elise HéonGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0003-4143-9311
Ajoy VincentGenetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID https://orcid.org/0000-0001-6446-3846

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transgenic mouse models are indispensable for dissecting disease mechanisms, yet their interpretability is frequently compromised by cryptic genomic alterations introduced during transgenesis. Thus, robust quality-control strategies are needed to elucidate integration architecture and evaluate model performance when such unintended events occur. Here, we applied unbiased whole-genome long-read sequencing using the PacBio Revio to investigate a mouse model exhibiting unexpected transgene silencing, originally designed to recapitulate autosomal-dominant hereditary macular dystrophy driven by the up-regulation of a

Identifiers

PMID42851758
PMCPMC13646464

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.