Evidence map›Paper›PMID 42851248›Full record

ArticleNeurogastroenterology and motility2026

Types of Neurons in the Myenteric Plexus of Human Gastric Corpus Identified by Multi-Layer Immunohistochemical Coding and Vagal Tracing.

M E Johnson, M Wee, B N Chen, A Humenick, R A Peterson, W P Yew, T J Hibberd, D A Wattchow, L Kow, T L Powley and 1 more

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In one paragraph

Article in Neurogastroenterology and motility, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

M E JohnsonCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0003-2653-3078
M WeeCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0002-6801-8974
B N ChenCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
A HumenickCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
R A PetersonCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.
W P YewCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0001-7436-0779
T J HibberdCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0002-3747-140X
D A WattchowCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0003-0180-7335
L KowCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0001-8028-5090
T L PowleyDepartment of Psychological Sciences and Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
S J H BrookesCollege of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0001-5635-0876

Funding

Mapping Stomach Autonomic Circuitry and Function for Neuromodulation of Gastric DisordersOT2OD023847 · OD · PURDUE UNIVERSITY · PI POWLEY, TERRY L. · 2016 to 2021
$17.6M
NIH HHS OD023847NIH HHS OT2 OD023847
6 · The paper itself

Abstract

backgroundEnteric neurons regulate motility, secretion, blood flow, and immune function of the gut. Characterizing neuron types is essential for understanding how the enteric nervous system works and how it goes wrong in disease.

methodsWe examined the myenteric plexus of the human gastric corpus from surgical specimens. Multiplexed immunohistochemistry assessed 15 markers, including pan-neuronal (HuC/D), neurotransmitter-related enzymes, nitric oxide synthase (NOS), choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), vesicular acetylcholine transporter (VAChT), the transmitter serotonin (by 5-HT accumulation), neuropeptides substance P (SP), enkephalin (ENK), calcitonin gene-related peptide (CGRP), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), calcium-binding proteins calbindin (Calb), calretinin (Calret) and the structural protein neurofilament 200 (NF200). These markers were applied to 1084 myenteric neurons from the gastric corpus of 7 individuals. Biotinamide tracing was used to label axons from intramural vagal nerve branches visible at the edge of the preparation. KEY

resultsTwelve classes of enteric neurons were distinguished by significant associations between immunohistochemical markers ("chemical coding"), including motor neurons and likely interneurons, secretomotor, and vasomotor neurons. Dense clusters of axonal varicosities ("baskets") labeled by VAChT, ENK, SP, or TH were abundant around ChAT+ myenteric cell bodies but disproportionately scarce around NOS+ cells. Vagal baskets labeled by biotinamide did not show an overall preference for either NOS+ or ChAT+ cells.

conclusionsMultiple types of human gastric myenteric neurons can be distinguished by their immunohistochemical profiles. Vagal efferent pathways are associated with all classes of myenteric neurons in the gastric corpus, whereas some enteric pathways likely exhibit selective connectivity with integrative potential.

Indexed as

Myenteric PlexusNeuronsStomachVagus NerveAdultAgedAged, 80 and overFemaleHumansImmunohistochemistryMaleMiddle Agedenteric nervous systemgastric corpushumanmyenteric plexusvagus nerve

Identifiers

PMID42851248
PMCPMC13650572

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.