Evidence map›Paper›PMID 42851083›Full record

ArticleTransboundary and emerging diseases2026

Genetic Diversity and Biological Characteristics of a Novel Feline Calicivirus Strain Isolated From Zoo-Captive South China Tiger (Panthera tigris amoyensis).

YuShan Ding, Niu Zhou, Hai Wang, Zhaoyang Liu, Fen Shan, Minting Liu, Yajiang Wu, Chen Wang, Mengna Lv, Wanqing Li and 9 more

Abstract read
PubMed Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

YuShan DingCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China, scau.edu.cn.ORCID https://orcid.org/0009-0009-5329-0359
Niu ZhouGuangzhou Zoo, Guangzhou, China.
Hai WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China, scau.edu.cn.
Zhaoyang LiuLuoyang City Landscape Gardening Center, LuoYang, China.
Fen ShanGuangzhou Zoo, Guangzhou, China.
Minting LiuGuangzhou Zoo, Guangzhou, China.
Yajiang WuGuangzhou Zoo, Guangzhou, China.
Chen WangGuangzhou Zoo, Guangzhou, China.
Mengna LvGuangzhou Zoo, Guangzhou, China.
Wanqing LiGuangzhou Zoo, Guangzhou, China.
Zujin ChenGuangzhou Zoo, Guangzhou, China.
Heng LiuLuoyang City Landscape Gardening Center, LuoYang, China.
Tianping LiLuoyang City Landscape Gardening Center, LuoYang, China.
Shiming PengGuangzhou Zoo, Guangzhou, China.
Xuanjiao ChenGuangzhou Zoo, Guangzhou, China.
Weijun XieGuangzhou Zoo, Guangzhou, China.
Xinyi CaoGuangzhou University, Guangzhou, China, gzhu.edu.cn.
Wu ChenGuangzhou Zoo, Guangzhou, China.ORCID https://orcid.org/0000-0002-4033-254X
Yongyi ShenSchool of Animal Sciences and Veterinary Medicine, Hainan University, Sanya, Hainan, China, hainu.edu.cn.ORCID https://orcid.org/0000-0001-7660-5522

Funding

Guangdong Major Project of Basic and Applied Basic Research 2020B0301030007Guangdong Provincial Key R&D Program 2022B1111040001Guangzhou National Research Laboratory GZNL2023A01001National Natural Science Foundation of China U24A20363
6 · The paper itself

Abstract

Feline calicivirus (FCV) is a common pathogen causing upper respiratory tract disease in domestic cats. Nonetheless, its threat and genetic features in endangered wild felids remain poorly understood. In this study, a novel FCV strain from a captive South China tiger (Panthera tigris amoyensis) was isolated, designated as FCV LYH1224. Viral RNA was detected in multiple tissues of the deceased tiger, including the heart, liver, spleen, lungs, and kidneys, suggesting broad tissue tropism and FCV contributed substantially to the fatal outcome. In vitro growth kinetics showed that the virus replicates efficiently in Crandell-Rees feline kidney (CRFK) cells. Whole-genome sequencing and phylogenetic analysis indicated that it was classified as genotype II (GII) and exhibited unique evolutionary lineages. Within GII, it clusters closely with the previously reported tiger-derived strain FCV-YH/16 but exhibits an independent branch in the VP1 gene tree, suggesting complex genetic variation among FCV strains from diverse wildlife origins. Sequence identity analysis indicated that FCV LYH1224 was genetically distant from vaccine strains F9 and 2280, with whole-genome nucleotide identity of 76.6% and 77.0%, respectively. Notably, the VP1-E region displayed the lowest identity, with nucleotide identity of 68.1% and 66.7%, respectively. Analysis of key amino acid residues revealed distinct substitutions within the neutralizing epitope (aa 445-451) and common mutations at virulence-related positions 452A and 492I, which were absent in reference strains, suggesting potential antigenic diversity and host-specific adaptations. This study provides a comprehensive genomic characterization of FCV strain in South China tigers, highlighting the genetic diversity and distinctive antigenic properties observed in this isolate compared with existing strains. The considerable genetic divergence from vaccine strains emphasizes the importance of ongoing surveillance and the inclusion of wildlife-derived strains in vaccine development strategies.

Indexed as

Caliciviridae InfectionsCalicivirus, FelineGenetic VariationTigersAnimalsAnimals, ZooChinaPhylogenygenetic diversitynovel feline calicivirusPanthera tigris amoyensisphylogenetic analysisVP1 protein

Identifiers

PMID42851083

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.