Evidence map›Paper›PMID 42848244›Full record

ArticleWorld journal of microbiology & biotechnology2026

Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health.

Mateus Rodrigues Alves, Nicolly Soares Ferreira, Max Sulivan Fontes, Mariana Fabri Lima, Marcelo Henrique Otenio, Mariana Drummond Costa Ignacchiti, Juliana Alves Resende

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Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mateus Rodrigues AlvesGraduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitario, no number, Guararema, Alegre, ES, 29500-000, Brazil.ORCID http://orcid.org/0009-0005-0723-0575
Nicolly Soares FerreiraGraduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Espírito Santo (UFES), Vitoria, Espírito Santo State, 29043-900, Brazil.ORCID http://orcid.org/0009-0001-2274-6300
Max Sulivan FontesDepartment of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil.ORCID http://orcid.org/0000-0003-3575-2184
Mariana Fabri LimaDepartment of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil.ORCID http://orcid.org/0009-0006-9233-819X
Marcelo Henrique OtenioEmbrapa Dairy Cattle - Brazilian Agricultural Research Corporation, Juiz de Fora, Brazil.ORCID http://orcid.org/0000-0002-6157-7861
Mariana Drummond Costa IgnacchitiDepartment of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES, 29500-000, Brazil.ORCID http://orcid.org/0000-0003-3768-949X
Juliana Alves ResendeGraduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitario, no number, Guararema, Alegre, ES, 29500-000, Brazil. juliana.resende@ufes.br.ORCID http://orcid.org/0000-0002-5476-3754

Funding

Fundação de Amparo à Pesquisa e Inovação do Espírito Santo FAPES; TO 326/2022
6 · The paper itself

Abstract

Antimicrobial-resistant bacteria in agricultural waste represent a critical One Health concern. Anaerobic digestion of livestock waste is widely promoted; however, the fate of antimicrobial resistance and biocide tolerance in full-scale systems remains unclear, constraining safe digestate reuse. This study assessed phenotypic and genotypic resistance in Escherichia coli-like enterobacteria isolated from effluent and biofertilizer in a full-scale dairy anaerobic digestion system. A total of 173 isolates were screened for antimicrobial resistance genes (ARGs), antimicrobial susceptibility, multidrug resistance (MDR), and biocide inhibition-zone responses. No diarrheagenic virulence genes were detected. At least one screened ARG was detected in 91.0% of effluent and 83.3% of biofertilizer isolates. qacE∆1 was the most frequent marker and occurred significantly more often in effluent (70.8%) than biofertilizer isolates (44.0%; q = 0.00291). Phenotypic resistance to at least one antimicrobial was observed in 27.0% and 34.5% of effluent and biofertilizer isolates, respectively, while MDR occurred in 2.2% and 9.5% (p = 0.0521). tet(a) carriage was strongly associated with tetracycline resistance (OR = 20.9; q < 0.001). H

Indexed as

DisinfectantsDrug Resistance, BacterialEnterobacteriaceaeAnaerobiosisAnimalsAnti-Bacterial AgentsCattleDairyingDrug Resistance, Multiple, BacterialGenes, BacterialGenotypeMicrobial Sensitivity TestsPhenotypeAnti-Bacterial AgentsDisinfectantsAnaerobic digestionAntimicrobial resistanceBiocideDairy cattleEnterobacterales

Identifiers

PMID42848244

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.