Evidence map›Paper›PMID 42848199›Full record

ArticlePlant cell reports2026

A miR65-MsbZIP11 post-transcriptional regulatory module coordinates saline-alkali stress tolerance in alfalfa (Medicago sativa L.).

Jiaqi Li, Rui Guo, Lei Liu, Lu Tang, Yarong Zhang, Jinyi Wang, Mengni Feng, Lei Ling, Changhong Guo

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jiaqi Li *Heilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Rui Guo *Heilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Lei Liu *Heilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Lu TangHeilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Yarong ZhangNational Technology Innovation Center for Prataculture, Hohhot, 010070, China.
Jinyi WangHeilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Mengni FengHeilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China.
Lei LingSchool of Biological Engineering, Daqing Normal University, Daqing, 163712, China.
Changhong GuoHeilongjiang Provincial Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin, 150500, China. kaku3008@hrbnu.edu.cn.ORCID http://orcid.org/0000-0001-8543-2942

Funding

Doctoral Research Startup Fund Project of Harbin Normal University XKB202307Natural Science Foundation of Heilongjiang Province No. PL2025C052the Fundamental Research Funds for Provincial Universities in Heilongjiang Province No. 2025-KYYWF-ZR0133the National Technology Innovation Center for Prataculture Special fund for innovation platform construction CCPTZX2024GJ02-002-03
6 · The paper itself

Abstract

key messageIntegrated multi-omics analyses reveal that novel miR65 represses MsbZIP11 and negatively regulates saline-alkali tolerance in alfalfa by impairing photosynthetic maintenance and disrupting redox homeostasis. MicroRNA-mediated post-transcriptional regulation plays an important role in plant adaptation to saline-alkali stress; however, the underlying regulatory modules remain poorly understood in alfalfa (Medicago sativa L.). Here, integrated profiling of small RNAs, the transcriptome, and degradome identified 175 stress-responsive miRNAs and 418 degradome-supported miRNA-mRNA interactions. We identified a previously uncharacterized miR65-MsbZIP11 module as a central regulatory node in the saline-alkali stress response. Reporter assays confirmed the novel miR65-directed repression of MsbZIP11. Silencing MsbZIP11 reduced stress tolerance, characterized by impaired photosynthetic performance, decreased soluble sugar accumulation, and elevated oxidative damage. miR65 overexpression produced similar phenotypes in hairy roots. Epistasis analysis further supported that miR65 acts upstream of MsbZIP11, as miR65 overexpression did not intensify stress sensitivity in an MsbZIP11-RNAi background. Together, these findings identify the miR65-MsbZIP11 module as a key post-transcriptional regulatory node coordinating saline-alkali stress tolerance in alfalfa by linking photosynthetic maintenance, redox homeostasis, and biomass stability.

Indexed as

AlkaliesMedicago sativaMicroRNAsPlant ProteinsGene Expression Regulation, PlantPhotosynthesisPlants, Genetically ModifiedRNA, PlantStress, PhysiologicalAlkaliesMicroRNAsPlant ProteinsRNA, PlantDegradome sequencinggenetic epistasisMedicago sativa L.miR65–MsbZIP11post-transcriptional regulationSaline-alkali stress

Identifiers

PMID42848199

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.