Mdivi-1 augments chlorpromazine's anticancer effect via modulation of mitochondrial dynamics, hallmarks of cancer-related cellular behavior, and oxidative stress.

Thoria Donia, Doha F Ismail, Samar S Alkafaas, Eiman Adly, Ashraf A Tabll, Mohamed Hessien

Abstract read

In one paragraph

Article in Medical oncology (Northwood, London, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Thoria DoniaMolecular Cell Biology Unit, Division of Biochemistry, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt. thoria_donia@science.tanta.edu.eg.ORCID http://orcid.org/0000-0002-1629-1290

Doha F IsmailMolecular Cell Biology Unit, Division of Biochemistry, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Samar S AlkafaasMolecular Cell Biology Unit, Division of Biochemistry, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Eiman AdlyMolecular Cell Biology Unit, Division of Biochemistry, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Ashraf A TabllImmunology Department, Egypt, Center for Research and Regenerative Medicine (ECRRM), Cairo, 11517, Egypt.

Mohamed HessienMolecular Cell Biology Unit, Division of Biochemistry, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt. mohamed.hessien@fulbrightmail.org.ORCID http://orcid.org/0000-0002-3782-1633

Funding

Academy of Scientific Research and Technology (ASRT), Egypt RESPECT-21-9999STDF - Science and Technology Development Fund C210000152

6 · The paper itself

Abstract

The multifaceted therapeutic potential of phenothiazines was previously documented. This study investigated the anticancer effect of chlorpromazine (CPZ) in hepatoma and breast cancer cell lines and further explored the implications of mitochondrial dynamics. To establish this aim, the cytotoxic and antimitotic effects of CPZ combined with the mitochondrial fission inhibitor (mdivi-1) were assessed in HepG2 and MDA-MB-231 cells using MTT and clonogenic assays, respectively. Apoptosis and nuclear changes were evaluated with Annexin V/PI, acridine orange/ethidium bromide, and DAPI staining. Moreover, cell migration was examined using a wound-healing assay and the expression of epithelial-mesenchymal transition (EMT) markers. Additionally, 2',7'-dichlorodihydrofluorescein diacetate staining was used to assess cellular ROS, whereas the expression of mitochondrial fusion- and fission-related genes was assessed by RT-PCR. The results addressed CPZ's cytotoxic effect in both cancer cell types. The IC

Indexed as

Antineoplastic AgentsChlorpromazineMitochondrial DynamicsOxidative StressQuinazolinonesApoptosisBreast NeoplasmsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionFemaleHep G2 CellsHumansMDA-MB-231 CellsReactive Oxygen Species3-(2,4-dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinoneAntineoplastic AgentsChlorpromazineQuinazolinonesReactive Oxygen SpeciesBreast cancerChlorpromazineHepatocellular carcinomaMdivi-1Mitochondrial fission

Identifiers

PMID42848096

DOI10.1007/s12032-026-03364-w

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