ArticleFEBS open bio2026
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells.
Article in FEBS open bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Originally developed for the field of physical chemistry, fluorescence fluctuation spectroscopy (FFS) has evolved into a family of methods to quantify concentrations, diffusion rates and interactions of fluorescently labeled molecules. Combining microscopy with the ability to measure molecules at the nanomolar level allows for the study of biological processes with high sensitivity even within the living cell. In this protocol, the principles of how to set up these types of experiments are described including calibration samples, challenges and specific cellular applications. The predecessor to FFS, fluorescence correlation spectroscopy (FCS) will be discussed which measures diffusion and concentrations at a user-defined point within the cell. Furthermore, an imaging variant, number and brightness analysis (N&B), will be shown that can be used to quantify molecular oligomerization. Overall, this protocol provides a foundation for using FFS and associated techniques to examine molecular dynamics within cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.