ArticleFrontiers in microbiology2026
A closed-loop tray bioreactor for online monitoring of mycelium-based composites.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
Mycelium-based composites are bio-derived structural materials produced by cultivating filamentous fungi through lignocellulosic substrates under solid-state fermentation conditions. Solid-state fermentation remains difficult to control because fungal growth is highly sensitive to local gradients in temperature, humidity, and gas composition. This study presents the design, construction, and experimental validation of a modular tray bioreactor for the controlled cultivation of mycelium-based composites. The system combines closed-loop feedback control of temperature and relative humidity with continuous monitoring of carbon dioxide and oxygen, online calculation of carbon dioxide evolution rate, programmable environmental trajectories, and synchronized multi-unit operation through a primary-secondary architecture. Performance was evaluated in two abiotic and two biotic experiments. Under abiotic conditions, the reactor provided stable regulation within a practical operating range of approximately 26-29°C and 25-50% relative humidity, with a steady-state temperature error below 0.5°C at the established setpoints and a root mean square error of 0.12°C during fixed-set-point operation. During biotic experiments with
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