Evidence map›Paper›PMID 42845382›Full record

ArticlebioRxiv : the preprint server for biology2026

Urothelial-lineage master transcription factor hub proteomics shows mechanisms impeding urothelial cancer cell differentiation.

Caroline Schuerger, Sudipta Biswas, Kwok Peng Ng, Lisa Cardone, Xiaorong Gu, Shinjini Ganguly, Rita Tohme, Arda Durmaz, Maximilian Stich, Daniel J Lindner and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Caroline SchuergerDepartment of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University.
Sudipta BiswasTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Kwok Peng NgTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Lisa CardoneTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Xiaorong GuTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Shinjini GangulyTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Rita TohmeTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Arda DurmazTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Maximilian StichDepartment of Pediatrics I, Heidelberg University, Heidelberg, Germany.
Daniel J LindnerTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Babal JhaTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.
Omar Y MianDivisions of Human Biology and Radiation Oncology, Fred Hutch Cancer Center, Seattle, WA.
Yogen SaunthararajahTranslational Hematology and Oncology Research Department, Lerner Research Institute, Cleveland Clinic.

Funding

TUMOR METABOLISM PROGRAMP30CA043703 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI MARK R CHANCE · 1987 to 2026
$142.3M
Targeting Enzymatic Regulation of Fetal Hemoglobin RepressionP01HL146372 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ENGEL, JAMES DOUGLAS · 2019 to 2023
$10.9M
Translational and Clinical Trial Correlates CoreU54CA274513 · NCI · CLEVELAND CLINIC LERNER COM-CWRU · PI Timothy An-thy Chan · 2022 to 2026
$9.3M
Imaging Liver Cancer ProliferationR01CA204373 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI LEE, ZHENGHONG, SAUNTHARARAJAH, YOGEN · 2016 to 2021
$1.8M
Sox9 signaling in lung adenocarcinomaR01CA190578 · NCI · RBHS -CANCER INSTITUTE OF NEW JERSEY · PI PINE, SHARON R. · 2014 to 2018
$1.7M
PerkinElmer IVIS Spectrum CTS10OD018205 · OD · CLEVELAND CLINIC LERNER COM-CWRU · PI DRAZBA, JUDY · 2015 to 2015
$600k
Operationalizing DNMT1-Targeting to Treat Chemorefractory Pancreatic CancerR21CA263430 · NCI · CLEVELAND CLINIC LERNER COM-CWRU · PI SAUNTHARARAJAH, YOGEN · 2022 to 2023
$409k
NCI NIH HHS P30 CA043703NCI NIH HHS R01 CA190578NCI NIH HHS R01 CA204373NCI NIH HHS R21 CA263430NCI NIH HHS U54 CA274513NHLBI NIH HHS P01 HL146372NIH HHS S10 OD018205
6 · The paper itself

Abstract

Urothelial cancer (UC) cells of the luminal subtype exhibit partial, incomplete differentiation towards umbrella cells that line bladder lumen, seen by morphology and gene expression. Differentiation is stalled even though the cells express master transcription factors (MTFs) that drive luminal urothelial differentiation, e.g., FOXA1 and CEBPB, at levels seen in normal differentiated urothelium. We therefore analyzed the FOXA1/CEBPB MTF hub by mass spectrometry. SWI/SNF coactivator complex (CoA) components, e.g., SMARCA4, ARID1A, that read the epigenetic activation mark histone 3 lysine 27 acetylation (H3K27ac) and use ATP-hydrolysis to 'open' chromatin, were the most abundant proteins pulled-down with FOXA1/CEBPB. However, genes for these and other CoA, e.g., CREBBP, EP300 that write H3K27ac, were mutated/deleted in >95% of UCs in clinical series. Also contained in the hub were corepressors (CoR) that erase H3K27ac and 'close' chromatin, e.g., HDAC1, CHD4 - genes for these CoR were recurrently gained in UCs. Chromatin analyses showed H3K27ac-centered remodeling was needed to activate umbrella but not constitutively accessible cell growth/division/housekeeping genes. Restoring ARID1A into

Indexed as

ARID1Abladder cancerCHD4coactivatorcorepressordifferentiationDNMT1epigeneticsFOXA1umbrella cellUrothelial cancer

Identifiers

PMID42845382
PMCPMC13640726

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.