ArticleFungal biology and biotechnology2026
Beyond carbon dioxide: decoupling headspace and buffering reveals a bicarbonate requirement for anaerobic gut fungi growth.
Article in Fungal biology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundAnaerobic gut fungi (Neocallimastigomycota) are major lignocellulose degraders in herbivore gastrointestinal tracts, but reproducible axenic cultivation remains difficult because conventional serum-bottle systems intrinsically couple CO
resultsComparison of CO
conclusionsHeadspace composition and inorganic carbon availability are key determinants of anaerobic gut fungal cultivation. The results indicate that bicarbonate supports sustained anaerobic gut fungal activity beyond extracellular pH stabilization and cannot be functionally replaced by the tested organic carboxylates. Decoupling gas phase, buffering chemistry, and pH control provides a more interpretable and reproducible cultivation framework for physiological studies and biotechnological application of anaerobic gut fungi.
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