Evidence map›Paper›PMID 42840676›Full record

ArticleRSC chemical biology2026

Genetically encoded dinitrobenzyl photostabilizer for enhancing the photostability of fluorescent proteins.

Guihua Li, Xia Wang, Jiben Xue, Jie Wang

Abstract read
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Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Guihua LiDepartment of Chemistry, College of Science, Guangdong Provincial Key Laboratory of Catalysis, Guangming Advanced Research Institute, Southern University of Science and Technology Shenzhen 518055 China.
Xia WangDepartment of Chemistry, College of Science, Guangdong Provincial Key Laboratory of Catalysis, Guangming Advanced Research Institute, Southern University of Science and Technology Shenzhen 518055 China.
Jiben XueDepartment of Chemistry, College of Science, Guangdong Provincial Key Laboratory of Catalysis, Guangming Advanced Research Institute, Southern University of Science and Technology Shenzhen 518055 China.
Jie WangTaizhou Research Institute, Southern University of Science and Technology Taizhou 318001 China wangjie@sustech.edu.cn.ORCID https://orcid.org/0000-0001-7602-5050

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescent proteins (FPs) are essential tools for biological imaging, but their application in long-term and super-resolution microscopy is often limited by photobleaching. Inspired by "self-healing" organic dyes, we report a genetically encoded intramolecular photostabilization strategy to improve the ability of fluorescent proteins to resist photobleaching. We designed a dinitrobenzene-based unnatural amino acid (UAA), 2-NBAK, to function as an efficient photostabilizer. Using an engineered orthogonal translation system, we performed a systematic spatial scan of 192 insertion sites across the mNeonGreen (mNG) β-barrel. Our screening revealed that optimal mNG-2-NBAK variants at specific outward-pointing side chains are proposed to quench the triplet state without disrupting the chromophore emission spectra.

Identifiers

PMID42840676
PMCPMC13640607

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.