Evidence map›Paper›PMID 42840113›Full record

ArticleFrontiers in microbiology2026

Monoclonal antibody 1B9 against the African swine fever virus viroporin pB169L: molecular characterization and epitope mapping.

Zhiying Cui, Jianing Feng, Miaomiao Ye, Qin Wen, Peng Gao, Qiongqiong Zhou, Yongning Zhang, Lei Zhou, Xinna Ge, Xin Guo and 2 more

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Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Zhiying Cui *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jianing Feng *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Miaomiao YeState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Qin WenState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Peng GaoState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Qiongqiong ZhouState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yongning ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Lei ZhouState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xinna GeState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xin GuoState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jun HanState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Hanchun YangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The African swine fever virus (ASFV) viroporin pB169L is a conserved membrane protein, but well-defined reagents for its detection and molecular characterization remain limited. Methods: A soluble recombinant antigen comprising the non-transmembrane regions of pB169L was used to generate a mouse monoclonal antibody (mAb), designated 1B9. The antibody specificity was characterized by immunoblotting and immunofluorescence assays. The expression and subcellular localization of pB169L were examined in ASFV-infected WSL-R4 cells and primary porcine alveolar macrophages. The variable regions of 1B9 were sequenced, and its epitope was mapped using truncated antigenic fragments. Results: The 1B9 antibody belonged to the IgG1 kappa subclass and recognized pB169L by immunoblotting and immunofluorescence at a working dilution of 1:2,000. In ASFV-infected cells, pB169L was detectable by immunofluorescence from 6 h post-infection and by Western blot from 12 h post-infection, with progressive accumulation in cytoplasmic viral factories together with p54. Two immunoreactive species of approximately 18 and 21 kDa were consistently observed. Variable-region sequencing defined the VH/VL gene usage and complementarity-determining regions of 1B9. Epitope mapping identified a linear epitope at residues Discussion: Thus, 1B9 provides a useful reagent for studying the expression, localization, and antigenic properties of pB169L.

Indexed as

African swine fever virusmonoclonal antibodypB169Lviral factoryviroporin

Identifiers

PMID42840113
PMCPMC13638613

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