Evidence map›Paper›PMID 42840083›Full record

ArticleFrontiers in genome editing2026

Genome edited sheep generated by zygote electroporation of prime editor ribonucleoproteins.

Zhixuan Yu, Junjin Li, Menghao Liu, Qi Qi, Zengyi Duan, Ziyu Yang, Boyuan Guan, Jiawang Chen, Jian Hou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in genome editing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhixuan YuState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Junjin LiState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Menghao LiuState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Qi QiState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Zengyi DuanState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Ziyu YangState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Boyuan GuanState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Jiawang ChenState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.
Jian HouState Key Laboratory of Animal Biotech Breeding and College of Biological Science, China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3 RNP formulation comprising PE2 protein, an epegRNA, and a nicking sgRNA into zygotes. We first validated functional PE2 protein and screened highly efficient enhanced pegRNAs (epegRNAs) targeting two high-value traits, including short-tail (

Indexed as

genome editingprime editingribonucleoproteinsheepzygote electroporation

Identifiers

PMID42840083
PMCPMC13638620

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.