ArticleFrontiers in pharmacology2026
Integrative proteomic and metabolomic analysis reveals that ouabain inhibits DLBCL proliferation by reprogramming arginine-driven metabolic networks.
Article in Frontiers in pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma (NHL), with an increasing incidence in recent years. Ouabain, a cardiotonic steroid, has demonstrated anti-tumor effects in various cancers including DLBCL, but its mechanism of action is not yet fully understood. We first used liquid chromatography-tandem mass spectrometry (LC-MS) for proteomics and metabolomics to analyze changes in DLBCL cells with ouabain administration. The results revealed significant alterations in the abundance of 303 proteins and 206 metabolites between the ouabain-treated and control groups. Proteins like MYC, RAD51, AURKB, CENPF, and RRM2 were downregulated, along with metabolism-related proteins ARG2, OAT, and TYMS. Metabolite levels including glutamate, glutathione, ornithine, and taurine were significantly altered. The integrated analysis revealed that arginine metabolism serves as a central hub, coordinating changes in glutathione metabolism and nucleotide biosynthesis, contributing to increased oxidative stress, replication stress, and growth inhibition. These findings suggest that ouabain disrupts arginine-driven metabolic network to alter redox balance and inhibit tumor cell proliferation, providing a promising therapeutic rationale for targeting metabolic vulnerabilities in DLBCL.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.