Evidence map›Paper›PMID 42834324›Full record

ReviewHormones (Athens, Greece)2026

Effects of calcitriol (1,25(OH)₂D₃) on endothelial-derived factors implicated in atheromatous plaque vulnerability.

Narjes Nasiri-Ansari, Aspasia Rizou, Christina-Maria Flessa, Manpal S Randeva, Eliana Spilioti, Maria-Eleni Chondrogianni, Lisa Dannenberg, Athanasios G Papavassiliou, Eva Kassi

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In one paragraph

Review in Hormones (Athens, Greece), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Narjes Nasiri-Ansari *Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece.
Aspasia Rizou *Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece.
Christina-Maria FlessaDepartment of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece.
Manpal S RandevaWarwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, CV2 2DX, UK.
Eliana SpiliotiLaboratory of Toxicological Control of Pesticides, Scientific Directorate of Pesticides' Control and Phytopharmacy, Benaki Phytopathological Institute, Kifissia, Greece.
Maria-Eleni ChondrogianniDepartment of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece.
Lisa DannenbergDepartment of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Athanasios G PapavassiliouDepartment of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece.
Eva KassiDepartment of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias Str., Goudi, Athens, TK, 11527, Greece. ekassi@med.uoa.gr.ORCID http://orcid.org/0000-0001-7491-2297

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAtherosclerosis progresses from endothelial dysfunction to arterial plaque vulnerability, rupture, erosion, and calcification. Vitamin D deficiency is an independent cardiovascular risk factor associated with advanced atherosclerotic disease. This study investigated whether calcitriol (1,25(OH)₂D₃), the active hormonal form of vitamin D, modulates the expression of MMP-2, MMP-9, TIMP-1, TIMP-2, MCP-1, RANK, RANKL, and OPG in human aortic endothelial cells (HAECs).

methodsCells were treated with calcitriol alone or in combination with TNF-α. Gene expression was assessed by qPCR, MCP-1 and OPG protein levels were measured by ELISA, and MMP activity by zymography.

resultsCalcitriol did not significantly affect basal MMP-2, MMP-9, TIMP-1, or TIMP-2 expression but reduced MCP-1 mRNA at 10⁻⁸ M. In TNF-α-activated cells, pre-incubation of cells with calcitriol decreased MMP-9 and MCP-1 expression, restored TIMP-2 levels, elevated the pro-/active-MMP-2 ratio and increased OPG mRNA expression, although this transcriptional effect was not accompanied by a corresponding increase in OPG protein levels.

conclusionThe effects of calcitriol on human aortic endothelial cells suggest that calcitriol modulates inflammatory and extracellular matrix-remodeling markers in TNF-α-stimulated human aortic endothelial cells. Whether these changes contribute to plaque stability requires confirmation in more complex experimental models.

Indexed as

1,25(OH)₂D₃AtherosclerosisCalcitriolHuman aortic endothelial cells (HAECs)InflammationVitamin D

Identifiers

PMID42834324

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.