Evidence map›Paper›PMID 42833214›Full record

ArticleMolecular cell2026

DND1-NANOS3 shapes the PGC transcriptome via a heptanucleotide sequence in mRNA 3' UTRs.

Masataka Suzawa, Chen Qiu, Ahsan H Polash, Misaki Yamaji, Alexis A Jacob, Wataru Horikawa, Eva M Farroha, Murchana Barua, Xuesong Feng, Chengyu Liu and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Masataka SuzawaRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Chen QiuEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA.
Ahsan H PolashRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Misaki YamajiDepartment of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Alexis A JacobRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Wataru HorikawaDepartment of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; RNA Biology Laboratory, National Cancer Institute, NIH, Frederick, MD 21702, USA.
Eva M FarrohaRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Murchana BaruaRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Xuesong FengLaboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Chengyu LiuTransgenic Core, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
Jason G WilliamsEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA.
Davide RandazzoLight Imaging Section, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Vittorio SartorelliLaboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA.
Eugene ValkovRNA Biology Laboratory, National Cancer Institute, NIH, Frederick, MD 21702, USA.
Masashi YamajiDepartment of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. Electronic address: m.yamaji@ono-pharma.com.
Traci M Tanaka HallEpigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA. Electronic address: hall4@niehs.nih.gov.
Markus HafnerRNA Molecular Biology Group, National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH, Bethesda, MD 20892, USA. Electronic address: markus.hafner@nih.gov.

Funding

Structural Studies Of Post-Transcriptional Gene RegulationZIAES050165 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI HALL, TRACI M · 2009 to 2025
$45.7M
Crystallographic studies of macromolecular structuresZICES102645 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI PEDERSEN, LARS · 2018 to 2025
$12.6M
Mass Spectrometry QuantitationZICES103005 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI WILLIAMS, JASON · 2018 to 2025
$9.2M
Integration of the results from systems-level determination of cis-acting elements into high-resolution maps of posttranscriptional regulatory events.ZIAAR041205 · NIAMS · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · PI HAFNER, MARKUS · 2016 to 2025
$5.5M
The role of AU-rich element binding proteins in shaping target mRNA expressionZIAAR041200 · NIAMS · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · PI HAFNER, MARKUS · 2014 to 2023
$5.1M
Structural Studies Of Post-transcriptional Gene RegulationZ01ES050165 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI HALL, TRACI M · 1998 to 2008
$5.0M
Intramural NIH HHS Z01 ES050165Intramural NIH HHS ZIA AR041200Intramural NIH HHS ZIA AR041205Intramural NIH HHS ZIA ES050165Intramural NIH HHS ZIC ES102645Intramural NIH HHS ZIC ES103005
6 · The paper itself

Abstract

Primordial germ-cell survival requires joint function of the RNA-binding proteins (RBPs) DND1 and NANOS3, yet how they collaborate has not been established. Here, we discover a paradigm of "two-factor authorization," where RBPs with low (DND1) or no (NANOS3) sequence specificity jointly repress RNAs via a unique high-information-content motif. Tandem PAR-CLIP showed that a DND1-NANOS3 complex represses mRNAs of dozens of key epigenome and cell-cycle regulators, recognizing a heptamer (AUGAAUU) in their 3' UTRs. Genome editing of this element relieved DND1-NANOS3-mediated repression of CDK1 in vivo. A high-resolution structure revealed a continuous RNA-binding surface formed by DND1 and NANOS3, and biochemical analysis indicated that a NANOS3 intrinsically disordered region contributes to high affinity and sequence specificity of the complex. RBPs are typically studied individually, thus missing new specificities imparted by ribonucleoprotein complex formation. Our study hints that two-factor authorization is an underappreciated regulatory mechanism for safeguarding against aberrant expression of post-transcriptional regulatory factors.

Indexed as

CLIPcross-linking and immunoprecipitationcrystal structurePAR-CLIPpost-transcriptional gene regulationribonucleoproteinsRNA-binding proteinRNPs

Identifiers

PMID42833214
PMCPMC13641165

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.