Evidence map›Paper›PMID 42830307›Full record

ArticleNature communications2026

Site-specific programming characterizes dynamic post-translational acetylation of histone H2B lysine 108 in mouse embryonic stem cells.

Fangni Chai, Qin Huang, Li Zhou, Yijia Ren, Yanping Zhong, Wei Cheng, Zhihong Xue, Haiyan Ren

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fangni Chai *Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.ORCID 0000-0002-8926-8622
Qin Huang *Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.ORCID 0009-0001-4751-9446
Li Zhou *Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
Yijia RenKey Laboratory of Birth Defects and Related Disease of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, PR China.
Yanping ZhongRespiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
Wei ChengRespiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.ORCID 0000-0002-7820-9774
Zhihong XueKey Laboratory of Birth Defects and Related Disease of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, PR China. xuez@scu.edu.cn.ORCID 0000-0003-3568-2964
Haiyan RenRespiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China. hyren@scu.edu.cn.ORCID 0000-0001-9995-3255

Funding

China Postdoctoral Science Foundation GZC20231839National Natural Science Foundation of China (National Science Foundation of China) 32271315
6 · The paper itself

Abstract

Dynamic histone modifications are critical for regulating stem cell fate. However, generalized strategies for site-specific programming of histone modifications profiling on unperturbed chromatin in embryonic stem cells (ESCs) remain challenging. In this study, we established a genetic code expansion-based platform in mouse ESCs (mESCs) for site-specific proteomic mapping and functional analysis within native chromatin. Using this platform, we revealed Rps19bp1 promotes Sirt1-mediated deacetylation of histone H2B lysine 108 acetylation (H2B-K108ac). Moreover, Site-specific-AcK revealed that H2B-K108ac induces the formation of H2B puncta and modulates chromatin accessibility. Functionally, elevation of H2B-K108ac via genetic code expansion and the loss of Rps19bp1 cause significant alterations in the expression of genes associated with mESC differentiation. Furthermore, Rps19bp1 deficiency promotes neural differentiation in mESC-derived teratoma. Our study develops a robust platform for linking specific histone acetylation with chromatin dynamics and cell fate determination, which lays a foundation for future exploration of additional histone PTMs in ESCs.

Indexed as

HistonesLysineMouse Embryonic Stem CellsProtein Processing, Post-TranslationalAcetylationAnimalsCell DifferentiationChromatinMiceProteomicsRibosomal ProteinsSirtuin 1ChromatinHistonesLysineRibosomal ProteinsSirt1 protein, mouseSirtuin 1

Identifiers

PMID42830307
PMCPMC13635217

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.