ArticleBMC plant biology2026
Evolutionary characteristics of the GAPDH gene family and functional characterization of TaGAPDH5 in wheat.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme that also plays important roles in plant responses to abiotic stresses. However, the evolutionary relationships of the GAPDH genes among wheat species with different ploidy levels remain poorly understood, and the key TaGAPDH members involved in the drought response of wheat have yet to be identified.
resultsIn this study, we identified 5, 6, 13, and 19 GAPDH proteins in Triticum urartu, Aegilops tauschii, Triticum turgidum, and Triticum aestivum with different ploidy levels. All identified GAPDH proteins harbored the conserved Gp_dh_N and Gp_dh_C domains and were phylogenetically assigned to four subfamilies: GAPA, GAPB, GAPC, and GAPCp. Gene structure and conserved motif analyses indicated overall conservation of the GAPDH family but clear divergence among subfamilies, with Motif 13 emerging as a characteristic motif unique to GAPB. Cis-element prediction revealed that the promoters of TaGAPDH genes contain numerous putative cis-acting elements associated with responses to drought and light. Transcriptomic profiling further verified that TaGAPDH genes exhibited tissue-specific expression patterns and were actively responsive to drought stress. Expression trend correlation analysis of drought-responsive transcriptomic data suggested a potential regulatory association between TaMYB-7D and TaGAPDH5. Consistently, transient dual-luciferase assays showed that TaMYB-7D activates the TaGAPDH5 promoter. Under drought stress, TaGAPDH5 overexpressing Arabidopsis thaliana lines displayed less intense DAB and NBT staining, higher soluble sugar contents, reduced wilting, and fewer dead leaves than wild type plants.
conclusionsCollectively, this study characterized GAPDH gene family members in wheat species of different ploidy levels and identified TaGAPDH5 as a potential drought tolerance candidate, providing a basis for further functional validation and possible application in drought tolerance breeding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.