ArticleInternational journal of microbiology2026
Molecular Characterization of Cocarriage Carbapenemase Genes in Clinical
Article in International journal of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Objectives: To determine the prevalence of carbapenem resistance and carbapenemase genes, characterize cocarriage of carbapenemase determinants, evaluate phenotypic and molecular detection methods, and identify factors associated with carbapenem-resistant Methods: A cross-sectional study was conducted from January to December 2023 at a tertiary-care teaching hospital in Chattogram, Bangladesh. Clinical specimens from 335 hospitalized patients were cultured, yielding 48 PCR-confirmed Results: Among 48 isolates, 27.1% were resistant to meropenem and 14.6% to colistin. Carbapenemase genes were detected in 20 (41.7%) isolates, with blaNDM being the predominant determinant (22.9%). Three isolates exhibited cocarriage of carbapenemase genes, including blaNDM+blaKPC, blaNDM+blaVIM, and blaNDM+blaOXA-48. Phenotypic testing identified carbapenemase activity in 14 isolates, whereas six additional gene-positive isolates were missed by mCIM. Phylogenetic analysis demonstrated close relatedness of detected carbapenemase genes to globally circulating variants. Conclusions: Carbapenem resistance in
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