Evidence map›Paper›PMID 42828749›Full record

ArticleSTAR protocols2026

Protocol for generating highly enriched sex-specific primary cortical astrocytes from individual mouse pups.

Sanarya Al-Jaf, En-Hung Ai, Julia A Wilson, Khaled S Abd-Elrahman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sanarya Al-JafDepartment of Anesthesiology, Pharmacology and Therapeutics, and Djavad Mowafaghian Centre for Brian Health, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada. Electronic address: sanarya.aljaf@ubc.ca.
En-Hung AiDepartment of Anesthesiology, Pharmacology and Therapeutics, and Djavad Mowafaghian Centre for Brian Health, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Julia A WilsonDepartment of Anesthesiology, Pharmacology and Therapeutics, and Djavad Mowafaghian Centre for Brian Health, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Khaled S Abd-ElrahmanDepartment of Anesthesiology, Pharmacology and Therapeutics, and Djavad Mowafaghian Centre for Brian Health, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Medical Sciences, College of Medicine and Health Sciences, Khalifa University, Abu Dhabi 127788, United Arab Emirates; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt. Electronic address: khaled.abdelrahman@ubc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for generating highly enriched primary cortical astrocytes from individual neonatal mouse pups. We describe steps for mechanically dissociating cortical tissue, culturing in coated flasks, and removing oligodendrocyte progenitor cells through mechanical shaking and selective pharmacological depletion of microglia. We then detail procedures for PCR-based analysis of tail-biopsy DNA to determine sex and genotype to genetically define astrocyte cultures. This protocol enables the investigation of sex- and genotype-specific mechanisms while avoiding the confounding effects of pooled tissue.

Indexed as

Cell BiologyMolecular BiologyNeuroscience

Identifiers

PMID42828749
PMCPMC13659762

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.