ArticleSTAR protocols2026
Protocol for imaging and quantitative analysis of yolk vesicle distribution and movement in syncytial Drosophila embryos.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
Drosophila embryos use maternally deposited yolk vesicles as their nutritional source during syncytial development. We present a protocol for imaging and quantifying yolk vesicle distribution and movement in mRFP-expressing embryos. We describe steps for imaging the embryo across nuclear division cycles, segmenting nuclei and yolk vesicles, and measuring density and size distribution. We detail procedures for analyzing cortical yolk vesicle movement with nuclei using particle image velocimetry and for quantifying net downward speed using particle tracking and a particle-conservation model. For complete details on the use and execution of this protocol, please refer to Thukral et al.
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