Evidence map›Paper›PMID 42826102›Full record

ArticleSTAR protocols2026

Protocol for brain and ventricle volume comparison after clearing and light-sheet microscopy in mice.

Ana Blas-Medina, Minh Son Phan, Samir Ali-Moussa, Nathalie Spassky, Julien Fernandes, Jean-Yves Tinevez, Aleksandra Deczkowska, Laetitia Travier

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ana Blas-MedinaBrain-Immune Communication Lab, Institut Pasteur, Université Paris Cité, Inserm U1224, 75015 Paris, France; PhD Program, École Doctorale Bio Sorbonne Paris Cité (BioSPC), Université de Paris Cité, 75005 Paris, France. Electronic address: ana.blas-medina@pasteur.fr.
Minh Son PhanImage Analysis Hub, C2RI Université Paris Cité, 75015 Paris, France.
Samir Ali-MoussaBrain-Immune Communication Lab, Institut Pasteur, Université Paris Cité, Inserm U1224, 75015 Paris, France; PhD Program, École Doctorale Bio Sorbonne Paris Cité (BioSPC), Université de Paris Cité, 75005 Paris, France.
Nathalie SpasskyInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, 75005 Paris, France.
Julien FernandesInstitut Pasteur, Université Paris Cité, Photonic Bio-Imaging Unit, Centre de Ressources et Recherches Technologiques (UTechS-PBI, C2RT), 75015 Paris, France.
Jean-Yves TinevezImage Analysis Hub, C2RI Université Paris Cité, 75015 Paris, France.
Aleksandra DeczkowskaBrain-Immune Communication Lab, Institut Pasteur, Université Paris Cité, Inserm U1224, 75015 Paris, France.
Laetitia TravierBrain-Immune Communication Lab, Institut Pasteur, Université Paris Cité, Inserm U1224, 75015 Paris, France. Electronic address: laetitia.travier@pasteur.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Analysis of brain and ventricular volumes provides insights into the physiological and pathological progression of brain development and aging, but accessible and cost-effective approaches are lacking. Here, we present a workflow to quantify relative brain and ventricular volumes in unsectioned cleared mouse brains following fluorescent detection of brain tissue and ventricle lining. We describe steps for brain harvesting, clearing, imaging by light-sheet microscopy, image resampling, and ventricle annotation, and comparing brains from adult, neonate, germ-free, and hydrocephalic mouse models.

Indexed as

Developmental biologyHealth SciencesMicroscopyModel OrganismsNeuroscience

Identifiers

PMID42826102
PMCPMC13659471

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.