ArticleFrontiers in nutrition2026
Extraction, purification, characterization, and
Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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9 authors.
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Abstract
Objective: To elucidate the anticoagulant effects of Methods: Crude polysaccharide (CC-1) was extracted by water extraction and ethanol precipitation, and then purified by papain-mediated deproteinization and dialysis to obtain polysaccharide CC-2. Chemical composition was analyzed using the phenol-sulfuric acid method, the meta-hydroxybiphenyl method, and a BCA protein assay kit. Molecular weight was determined by high-performance gel permeation chromatography (HPGPC). Monosaccharide composition was analyzed by PMP pre-column derivatization HPLC (PMP-HPLC). Structural features were characterized by Fourier transform infrared (FT-IR) and ultraviolet (UV) spectroscopy, and scanning electron microscopy (SEM). Results: The yield of CC-2 was 2.16%. Compared with CC-1, CC-2 showed significantly higher total sugar and uronic acid contents and a decreased protein content. The molecular weight of CC-2 was 5908.612 Da. Its monosaccharide composition consisted primarily of rhamnose, glucuronic acid, glucose, galactose, and xylose in a molar ratio of 5:1:4:11:4. FT-IR indicated the presence of acetyl groups and a furanose ring conformation. SEM revealed a microstructure composed of irregular lumps and spherical particles. Conclusion:
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