Evidence map›Paper›PMID 42822457›Full record

ArticleCurrent biology : CB2026

A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.

Manish Goyal, Tiana M Issa, Jonathan A Stefely, Kailash Venkatraman, Jason G McCoy, Stephanie S Lam, Michael Z Chen, Khanh Nguyen, Cameron Genxuan Lian, Pierre M Jean-Beltran and 9 more

Abstract read
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In one paragraph

Article in Current biology : CB, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Manish GoyalDepartment of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA.
Tiana M IssaDepartment of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Jonathan A StefelyDepartment of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Kailash VenkatramanDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Jason G McCoyDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Stephanie S LamDepartment of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Michael Z ChenDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Khanh NguyenBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cameron Genxuan LianBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Pierre M Jean-BeltranBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Michelle Y FryDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA.
Bridget E LuceDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA.
MitoCarta Tree of Life Consortium
Luke H ChaoDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA.
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Namrata D UdeshiBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Sarah E CalvoDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
Vamsi K MoothaDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA. Electronic address: vamsi_mootha@hms.harvard.edu.
John SamuelsonDepartment of Molecular and Cell Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA. Electronic address: jsamuels@bu.edu.

Funding

Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
Medical Scientist Training ProgramT32GM144273 · NIGMS · HARVARD MEDICAL SCHOOL · PI David Shumway Jones, Jacqueline A. Lees · 2022 to 2026
$14.7M
MOLECULAR IMMUNOLOGY AND TUMOR BIOLOGYT32CA009216 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI David Michael Langenau · 1985 to 2026
$10.6M
Establishing Acanthamoeba as a modern model parasite for infectious disease biology and comparative metabolism by defining mitochondrial proteins and pathwaysK08AI193194 · NIAID · MORGRIDGE INSTITUTE FOR RESEARCH, INC. · PI Jonathan Allan Stefely · 2025 to 2026
$358k
Howard Hughes Medical InstituteNCI NIH HHS T32 CA009216NIAID NIH HHS K08 AI193194NIGMS NIH HHS T32 GM007753NIGMS NIH HHS T32 GM144273
6 · The paper itself

Abstract

Giardia lamblia is a microaerophilic protozoan parasite that infects nearly 280 million people worldwide each year. Giardia lacks a mitochondrial genome and mitochondrial enzymes for oxidative phosphorylation. However, it retains mitochondrion-related organelles (MROs) that house an iron-sulfur cluster (ISC) assembly pathway. To date, 32 MRO proteins have been identified with high confidence. Purifying MROs is extremely challenging due to their small size and low abundance. Here, as part of the MitoCarta Tree of Life Consortium, we used large-scale culture, density gradient fractionation, affinity purification, data-independent acquisition mass spectrometry (DIA-MS), protein-correlation profiling, and confocal microscopy to generate the Giardia MitoCarta (GlMitoCarta) inventory. GlMitoCarta consists of 59 proteins, 27 of which are new and 34 with unknown function. Metronidazole unexpectedly upregulates many components of the MRO. Immunoprecipitation (IP)-MS analysis reveals that MROX6 and MROX13, proteins of unknown function, reciprocally interact and share an overlapping interactome related to ISC biology. Further, we identify MOMTiP-4 as a likely MRO inner membrane solute transporter that structurally resembles and functionally complements the yeast SLC25A carrier, Mtm1. The GlMitoCarta resource is freely available and should serve as a valuable foundation for systematic and mechanistic studies of MRO biology and Giardia pathogenesis.

Indexed as

Giardiairon-sulfur cluster assemblyMetamonadametronidazoleMitochondrion Related OrganelleMitoProteomeMOMTiP-4Mtm1perturbation profilingSLC25A39

Identifiers

PMID42822457
PMCPMC13643923

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.