Evidence map›Paper›PMID 42822347›Full record

ArticlePoultry science2026

Development and evaluation of bovine adenovirus vector-based avian influenza vaccine efficacy in poultry.

Shekoni Olaitan Comfort, Ekramy E Sayedahmed, Wen-Chien Wang, Raksha Suresh, Jennifer Schrock, Renick Wiltshire, Xingyu Wu, Alok Dhakal, Stephen A Akinola, Megan Tenney and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Shekoni Olaitan ComfortCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Ekramy E SayedahmedDepartment of Comparative Pathobiology, and Purdue Institute of Inflammation, Immunology and Infectious Disease, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Wen-Chien WangDepartment of Comparative Pathobiology, and Purdue Institute of Inflammation, Immunology and Infectious Disease, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Raksha SureshCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Jennifer SchrockCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Renick WiltshireCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Xingyu WuCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Alok DhakalCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Stephen A AkinolaCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Megan TenneyCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Khadijat AdefayeCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Saroj KhatiwadaCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Gowtham Avverahalli ShivalingaiahCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Patricia BoleyCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Mithilesh SinghCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Ramon Lagos ZegpiCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
Suresh K MittalDepartment of Comparative Pathobiology, and Purdue Institute of Inflammation, Immunology and Infectious Disease, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Gourapura J RenukaradhyaCenter for Food Animal Health, Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Wooster, OH, USA. Electronic address: gourapura.1@osu.edu.

Funding

Adenoviral Vector-based Pandemic Influenza VaccineR01AI059374 · NIAID · PURDUE UNIVERSITY WEST LAFAYETTE · PI SURESH K MITTAL · 2005 to 2026
$5.0M
NIAID NIH HHS R01 AI059374
6 · The paper itself

Abstract

Avian influenza virus (AIV) remains a persistent threat to global poultry health and production, necessitating the development of novel and effective vaccination strategies. This study evaluated the immunogenicity and protective efficacy of a newly developed bovine adenovirus-vector-based avian influenza virus (BAdV-AIV) vaccine expressing the hemagglutinin (HA) gene of H5N1 and the nucleoprotein (NP) gene of H7N9 AIV subtypes (BAdV - (H5N1)HA+(H7N9)NP). In our study, the candidate vaccine was administered via three mucosal routes: oral (OR), intranasal (IN), or intraocular (IO), using three different doses (10⁶, 10⁷, and 10⁸ pfu/bird) in a prime-boost vaccination regimen in broiler chickens. Immunological analyses revealed that high-dose vaccination, administered via the IO route induced a substantial upregulation of virus-specific systemic IgY, hemagglutination inhibition (HI) antibody titers, mucosal sIgA antibody responses in bile, trachea and intestines, and activation of various T-cell subsets (helper T cells, cytotoxic T cells, and γδ T lymphocytes). The quantitative PCR (qPCR) assay failed to detect a difference in the challenge H5N2 AIV genomic equivalent load in the vaccinated groups. A qualitative analysis for live challenge virus detection was performed by inoculating the tracheal swab fluid into embryonated chicken eggs and harvested allantoic fluid was tested by hemagglutination assay. Our data showed a lower percentage of tracheal samples yielding detectable live virus in the high-dose BAdV-(H5N1)HA+H7N9)NP AIV vaccine group, particularly following IO administration, compared with the other vaccination routes. Overall, the BAdV-(H5N1)HA+ (H7N9)NP vaccine elicited AIV-specific humoral, mucosal, and cellular immune responses and was associated with a reduced percentage of virus-isolation-positive tracheal samples, particularly following IO administration. These findings support the need for further evaluation of this vaccine platform for avian influenza control in poultry.

Indexed as

Avian influenza virusBovine adenovirus vaccineChickensMucosal deliveryVaccine

Identifiers

PMID42822347
PMCPMC13659499

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.