Hemolytic Activity and Quantitative Characteristic of Pneumolysin Depending on the Culture Medium of Streptococcus pneumoniae Strains.

E A Kurbatova, N F Gavrilova, I V Yakovleva, A E Zaytsev, An A Poddubikov, E S Petukhova, I B Semenova, Yu V Volokh, N O Vartanova

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Article in Bulletin of experimental biology and medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

9 authors.

E A KurbatovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia. kurbatova6162@yandex.ru.

N F GavrilovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

I V YakovlevaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

A E ZaytsevI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

An A PoddubikovN. V. Sklifosovskiy Institute of Clinical Medicine, Sechenov First Moscow State Medical University, Moscow, Russia.

E S PetukhovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

I B SemenovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

Yu V VolokhI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

N O VartanovaI. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The hemolytic activity of pneumolysin produced by Streptococcus pneumoniae depends on the culture medium used for bacterial growth. In brain-heart infusion broth, both the culture medium and bacterial cell supernatants from all strains induced erythrocyte lysis and contained pneumolysin, which was detected using sandwich ELISA based on IgM and IgG1-HRP monoclonal antibodies to native pneumolysin. In contrast, samples obtained from strains grown on blood agar exhibited virtually no hemolytic activity, despite the presence of pneumolysin at high concentrations. These findings suggest that the culture medium affects the production of functionally active pneumolysin by pneumococcal strains. The developed sandwich ELISA based on monoclonal antibodies allows detection of pneumolysin that has lost its hemolytic properties.

Indexed as

bacterial pneumolysinhemolysismonoclonal antibodiessandwich ELISAStreptococcus pneumoniae serotypes

Identifiers

PMID42821222

DOI10.3390/biom10071009

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