Evidence map›Paper›PMID 42821166›Full record

ArticleJournal of molecular histology2026

Glomerular epithelial protein-1 promotes reparative dentin formation in vivo.

Elina Pokharel, Moo-Keun Song, Bandana Rana, Yam Prasad Aryal, Tae-Young Kim, Ji-Youn Kim, Je-Hee Jang, Jae-Hee Lee, Mee-Seon Kim, Hitoshi Yamamoto and 8 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Elina Pokharel *Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Moo-Keun Song *Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Bandana RanaDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Yam Prasad AryalDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Tae-Young KimDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Ji-Youn KimDepartment of Dental Hygiene, College of Medical Science, Gachon University, Incheon, 21936, South Korea.
Je-Hee JangDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Jae-Hee LeeDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Mee-Seon KimDepartment of Pathology, School of Dentistry, Kyungpook National University, Kyungpook National University Hospital, Daegu, 41940, South Korea.
Hitoshi YamamotoDepartment of Histology and Developmental Biology, Tokyo Dental College, Tokyo, 101-0061, Japan.
Sung-Min HwangDepartment of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Chang-Hyeon AnDepartment of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Wern-Joo SohnDepartment of K-Beauty Business, College of K-BioHealth, Daegu Haany University, Gyeongsan, 38610, South Korea.
Seo-Young AnDepartment of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Sung-Jin ChoDepartment of Biological Sciences and Biotechnology, College of Natural Sciences, Chungbuk National University, Cheongju, 28644, South Korea.
Jung-Hong HaDepartment of Conservative Dentistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea.
Jae-Kwang JungDepartment of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea. widenmy@knu.ac.kr.
Jae-Young KimDepartment of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea. jykim91@knu.ac.kr.ORCID https://orcid.org/0000-0002-6752-5683

Funding

National Research Foundation of Korea NRF-2022R1I1A2063745National Research Foundation of Korea RS-2023-00252263
6 · The paper itself

Abstract

Glomerular epithelial protein-1 (Glepp1/PTPRO), a member of the R3 subtype family of receptor-type protein tyrosine phosphatases that, regulates cellular processes, like proliferation, differentiation, and apoptosis, involved in odontogenesis, with its overexpression causing thicker dentin. However, its in vivo impact during reparative dentin formation remains unexplored. This study evaluated an alternative therapeutic approach of enhancing natural dentin repair by overexpressing Glepp1 in an animal model. A pulp access cavity was prepared on the upper right first molar of eight-week-old male mice, followed by local delivery of Glepp1 adeno-associated virus (AAV) or phosphate-buffered saline (PBS). Hematoxylin and eosin (H&E) staining and immunohistochemistry were used to assess histological and cellular physiological changes after three and five days of treatment. Micro-CT and H&E analyses 42 days after AAV-mediated delivery revealed significantly enhanced dentin bridge formation and increased mineralized tissue deposition in the Glepp1 AAV-delivered group. Glepp1 AAV-delivered samples showed increased immunolocalization of reparative dentin-related markers, including Nestin, runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osteopontin (OPN) at days 3 and 5, suggesting enhanced mineralization and extracellular matrix formation. Furthermore, decreased tumor necrosis factor α (TNFα) and increased transforming growth factor β - 1 (TGF-β1) localization with enhanced cluster of differentiation 31 (CD31) immunostaining indicated inflammatory response modulation and improved vascularization. These findings highlight local Glepp1 overexpression using the AAV system as a viable strategy for dentin-pulp regeneration via modulation of cellular and molecular mechanisms.

Indexed as

DentinDentinogenesisDentin, SecondaryAnimalsDependovirusMaleMiceAAVDentin bridgeGlepp1Pulp cavityReparative dentin

Identifiers

PMID42821166

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.