Evidence map›Paper›PMID 42821120›Full record

ArticleBiodegradation2026

Genomic insights into the metabolic complementarity of a synthetic marine consortium for hydrocarbon degradation in coastal sand microcosms.

Karla Sofía Millán-López, Juan M Zurita-Artaloitia, Adolfo Gracia, Liliana Pardo-López

Abstract read
In one paragraph

Article in Biodegradation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Karla Sofía Millán-LópezLaboratorio de Biotecnología Marina, Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos, México.
Juan M Zurita-ArtaloitiaLaboratorio de Biotecnología Marina, Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos, México.
Adolfo GraciaInstituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico, México.
Liliana Pardo-LópezLaboratorio de Biotecnología Marina, Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos, México. liliana.pardo@ibt.unam.mx.ORCID https://orcid.org/0000-0002-8927-1733

Funding

Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México IG200223SECIHTI MADTEC-2025-M-60Secretaría de Ciencia, Humanidades, Tecnología e Innovación LANCO
6 · The paper itself

Abstract

Microbial consortia are essential for the degradation of complex hydrocarbon mixtures in coastal environments, yet the functional basis of consortium performance rarely resolved at strain level. In this study, we employed a Plackett-Burman experimental design to rationally assemble and optimize a synthetic consortium for the degradation of medium-grade crude oil in a sand-seawater microcosm. Six strains were selected: Stutzerimonas stutzeri CBC1 and CBC2, S. balearica GOM11, Pseudomonas sp. CBC3, Halomonas litopenaei GOM23 and Enterobacter kobei GOM24. After 45 days, the consortium removed 15.72% of total hydrocarbons relative to the abiotic control, with markedly higher fraction-specific removal determined by GC-MS: 94.7% of phenanthrene, 63.1% and 41.0% of the C8-C27 and C18-C27 aliphatic fractions respectively. Comparative genomic analysis revealed a non-uniform distribution of catabolic functions consistent with metabolic complementary. Stutzerimonas and Pseudomonas strains carried ring-hydroxylating dioxygenases (nah/nag-like clusters) together alkane-degrading alcohol/aldehyde dehydrogenases (alkJ, alkH), whereas H. litopenaei GOM23 and E. kobei GOM24 encoded alternative long-chain alkane-hydroxylating systems (ladA). GOM24 further carried an expanded repertoire of ABC transporters and oxidative-stress genes (ahpC/ahpF), consistent with a role in substrate uptake and stress tolerance rather than in primary ring cleavage. Enzymatic assays supported this partitioning, catechol 1,2-dioxygenase activity was confined to the same four strain that encoded the gene and was highest in CBC1 and GOM11 (0.0472 and 0.0443 U·mg⁻

Indexed as

BacteriaHydrocarbonsMicrobial ConsortiaSeawaterBacterial ProteinsBiodegradation, EnvironmentalGenome, BacterialGenomicsPetroleumSilicon DioxideBacterial ProteinsHydrocarbonsPetroleumSilicon DioxideHydrocarbon degradationMarine bacteriaPlackett–Burman designSynthetic consortium

Identifiers

PMID42821120
PMCPMC13630889

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.