Evidence map›Paper›PMID 42820038›Full record

ArticleChemical & biomedical imaging2026

An Open, Integrated Platform for Multiplexed Bioluminescence Microscopy.

Lorenzo Scipioni, Belen Torrado, Giulia Tedeschi, Lila P Halbers, Zachary R Torrey, Erin B Fuller, Francesco Fersini, Christoph Gohlke, Andrej Luptak, Jennifer A Prescher and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Lorenzo ScipioniLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Belen TorradoLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Giulia TedeschiLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Lila P HalbersDepartment of Pharmaceutical Sciences, University of California, Irvine, California 92697, United States.
Zachary R TorreyDepartment of Chemistry, University of California, Irvine, California 92617, United States.ORCID https://orcid.org/0000-0002-6854-3875
Erin B FullerDepartment of Chemistry, University of California, Irvine, California 92617, United States.ORCID https://orcid.org/0009-0006-8711-5960
Francesco FersiniCentre de Recherche en Cancérologie de Toulouse, Toulouse 31100, France.
Christoph GohlkeLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Andrej LuptakDepartment of Pharmaceutical Sciences, University of California, Irvine, California 92697, United States.ORCID https://orcid.org/0000-0002-0632-5442
Jennifer A PrescherDepartment of Pharmaceutical Sciences, University of California, Irvine, California 92697, United States.ORCID https://orcid.org/0000-0002-9250-4702
Michelle A DigmanLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.ORCID https://orcid.org/0000-0003-4611-7100

Funding

TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
NIGMS NIH HHS P41 GM103540
6 · The paper itself

Abstract

We report an imaging package to democratize all-in-one bioluminescence and fluorescence microscopy. The platform comprises three tools: PhasorViewer, a visualization suite to design experiments and identify optimal probe combinations; PhasorScope, an open-source, cost-effective microscopy framework to upgrade conventional microscopes; and PhasorAnalysis, a dedicated, user-friendly analysis pipeline to quantify phasor imaging data. We demonstrate the utility of the platform for multiplexed, simultaneous fluorescence and bioluminescence imaging with readily accessible optical reporters.

Indexed as

bioluminescencefluorescencemicroscopymultiplexingphasors

Identifiers

PMID42820038
PMCPMC13625740

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.