Evidence map›Paper›PMID 42819911›Full record

ArticleRSC chemical biology2026

High-throughput droplet screening of filamentous fungi on solid substrates.

Chiara Leal Alves, Jeremy Z Wu, Elham Khanjani, Adiphol Dilokpimol, Zhiyang Deng, Biplu Prajapati, Niloufar Rahbari, Antti P Aalto, Mari Valkonen, Steve C C Shih

Abstract read
In one paragraph

Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chiara Leal AlvesDepartment of Electrical and Computer Engineering, Concordia University Montréal Québec Canada steve.shih@concordia.ca.
Jeremy Z WuCentre for Applied Synthetic Biology, Concordia University Montréal Québec Canada.
Elham KhanjaniCentre for Applied Synthetic Biology, Concordia University Montréal Québec Canada.
Adiphol DilokpimolVTT Technical Research Centre of Finland Ltd Tekniikantie 21 Espoo Finland mari.valkonen@vtt.fi.
Zhiyang DengDepartment of Electrical and Computer Engineering, Concordia University Montréal Québec Canada steve.shih@concordia.ca.
Biplu PrajapatiVTT Technical Research Centre of Finland Ltd Tekniikantie 21 Espoo Finland mari.valkonen@vtt.fi.
Niloufar RahbariCentre for Applied Synthetic Biology, Concordia University Montréal Québec Canada.
Antti P AaltoVTT Technical Research Centre of Finland Ltd Tekniikantie 21 Espoo Finland mari.valkonen@vtt.fi.
Mari ValkonenVTT Technical Research Centre of Finland Ltd Tekniikantie 21 Espoo Finland mari.valkonen@vtt.fi.
Steve C C ShihDepartment of Electrical and Computer Engineering, Concordia University Montréal Québec Canada steve.shih@concordia.ca.ORCID https://orcid.org/0000-0003-3540-0808

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Droplet-based microfluidics has been frequently used for high-throughput single-cell microbial screening, where individual cells are encapsulated into nanoliter or picoliter-sized droplets and sorted based on measurable phenotypes such as fluorescent proteins or enzyme activity using fluorescence or absorbance-based substrates. However, using droplet microfluidics with filamentous fungi poses a unique challenge: fungal strains tend to escape from droplets as their hyphae grow, causing individual droplets to merge and eventually burst which prevents measuring individual phenotypes. In our work, we demonstrate a strategy to robustly encapsulate and retain spores of filamentous fungi within water-in-oil droplets by incorporating solid substrates into the droplet microenvironment. Using this approach, we show for the first time the encapsulation and growth of two model filamentous fungi species in droplets -

Identifiers

PMID42819911
PMCPMC13625276

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.