Introduction: Honey-based biomaterials have gained increasing interest as topical strategies for wound-related applications because of their antimicrobial, antioxidant, and tissue-supportive properties. However, the direct use of raw honey is limited by its viscosity, variability, and lack of standardized delivery systems. This study aimed to perform a preliminary Methods: Eight Chilean honey samples with varying contributions of Quillay ( Results: All honey samples met basic physicochemical quality criteria. CHH formulations did not reduce fibroblast metabolic activity, whereas CHH-50% and CHH-100% significantly increased resazurin reduction compared with untreated control cells. Raw honey significantly increased nitrite accumulation, whereas CHH formulations did not increase nitrite levels under the experimental conditions tested. In agar diffusion assays, CHH produced larger and more homogeneous inhibition zones than raw honey against Discussion: These findings support CHH as a biocompatible honey-derived formulation with improved antibacterial performance compared with raw honey under