Development and Validation of a Simultaneous RP-HPLC Method for the Estimation of Ferrous Fumarate and Ascorbic Acid for Pharmaceutical Quality Control.

Vinay Kumar, Muralidhar Pisay, Anoushka Mukharya, Komal Rana, Sanjay Bharati, Muddukrishna Badamane Sathyanarayana, Srinivas Mutalik

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Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Vinay KumarDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.

Muralidhar PisayDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.

Anoushka MukharyaDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.

Komal RanaManipal - Government of Karnataka Bioincubator, Advanced Research Centre, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.

Sanjay BharatiDepartment of Nuclear Medicine, Manipal College of Health Professions, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.ORCID https://orcid.org/0000-0002-3697-2632

Muddukrishna Badamane SathyanarayanaDepartment of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.ORCID https://orcid.org/0000-0001-6447-5759

Srinivas MutalikDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.ORCID https://orcid.org/0000-0002-0642-1928

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6 · The paper itself

Abstract

Our aim was to develop an accurate, precise, and reliable RP-HPLC method for estimating ferrous fumarate and ascorbic acid in pro-liposomes. The aim was to make the analytical method simple, environmentally friendly, and accurate. To measure ferrous fumarate (FF) and ascorbic acid (AA), an isocratic method was used in an HPLC, with 220 and 245 nm detection, using phosphate buffer (pH 3.0) and methanol (95:5). It was found that AA and FF were retained for 6.4 and 8.3 min, respectively. A linear relationship was observed between 0.1 and 50 μg/mL for both ferrous fumarate (

Identifiers

PMID42819094

DOI10.1021/acsomega.6c02561

PMCPMC13624945

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