ArticleACS omega2026
Coengineering Vapor-Deposited Polycationic Microenvironments and Immobilization Strategy to Enhance β‑Galactosidase Kinetics at Acidic pH.
Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
The industrial application of enzymes, such as β-galactosidase (LacZ) for processing acid whey, is often constrained by their poor activity at acidic pH. This work addresses this limitation by covalently immobilizing LacZ on copolymer thin films synthesized by initiated chemical vapor deposition (iCVD) containing 2-(dimethylamino)-ethyl methacrylate (DMAEMA), which becomes positively charged at acidic pH. Chemically analogous self-assembled monolayer (SAM) supports served as a solution-synthesized benchmark. We systematically investigated immobilization strategiesrandom immobilization (RI) vs directed immobilization (DI, via SpyCatcher/SpyTag)and support properties (polycationic content, cross-linking degree, support thickness, and synthesis route: iCVD vs SAM), quantifying their effects on LacZ activity and kinetic parameters. Generally, our results indicate that LacZ immobilized via DI outperformed RI across support properties under wide pHs (4-8), mainly by enhancing the LacZ's intrinsic turnover number (
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