Evidence map›Paper›PMID 42818571›Full record

ArticleFrontiers in cellular and infection microbiology2026

Development and evaluation of a recombinase-aided amplification-lateral flow dipstick assay for rapid and sensitive detection of

Shu Wang, Yingkai Zhu, Yuxin Wang, Fang Wang, Kexun Lian, Xuemei Sun, Zexuan Wang, Xinghong Ding, Yali Han, Ruinan Wang and 2 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shu WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yingkai ZhuCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yuxin WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Fang WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Kexun LianCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Xuemei SunXinjiang Taikun Group Co., Ltd. Innovation Research Institute, Xinjiang Taikun Group Co., Ltd., Changji, Xinjiang, China.
Zexuan WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Xinghong DingCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yali HanCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Ruinan WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yaocheng CuiCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Huiyong XuanCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Methods: According to the design rules for RAA primers and exo-probes, specific primers and a probe were designed targeting the conserved region of the MS Results: The RAA reaction was completed within 20 min at a constant temperature of 37 °C, and amplification products were visually read within 3-5 min using a lateral flow dipstick without sophisticated equipment. The total detection time, including the DNA extraction step, is completed within 40 min. Methodological validation revealed that the RAA-LFD assay exhibited high specificity, with no cross-reactivity observed against other common avian pathogens. The assay also showed satisfactory repeatability and high sensitivity, with a limit of detection of 2.63×10 Conclusion: In summary, the established RAA-LFD assay is rapid, user-friendly, highly specific, and sensitive. To further improve the RAA-LFD-MS assay for routine clinical and field use, future efforts should prioritize the optimization of rapid DNA extraction, accessible temperature-control devices, and reagent lyophilization.

Indexed as

Mycoplasma InfectionsMycoplasma synoviaeNucleic Acid Amplification TechniquesPoultry DiseasesRecombinasesAnimalsBacterial ProteinsChickensDNA, BacterialDNA PrimersLectinsRapid Diagnostic TestsReproducibility of ResultsSensitivity and SpecificityBacterial ProteinsDNA, BacterialDNA PrimersLectinsRecombinasesVlhA protein, Mycoplasma synoviaelateral flow dipstickMycoplasma synoviaenucleic acid detectionrapid diagnosisrecombinase-aided amplification

Identifiers

PMID42818571
PMCPMC13623553

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